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A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System
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JoVE Journal Medicina
A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

A Drosophila In Vivo Injury Model for Studying Neuroregeneration in the Peripheral and Central Nervous System

DOI:
10.3791/57557-v

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09:55 min

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May 05, 2018

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, , ,
1Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia, 2Department of Pathology and Laboratory Medicine,University of Pennsylvania

Capítulos

  • 00:04Título
  • 01:08Collecting Larvae
  • 02:11Microscope Set Ups
  • 04:18Two-photon Injury
  • 07:40Results: Axon Regeneration in the PNS
  • 08:49Conclusion

Summary

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Here, we present a protocol using the Drosophila sensory neuron - dendritic arborization (da) neuron injury model, which combines in vivo live imaging, two-photon laser axotomy/dendriotomy, and the powerful fly genetic toolbox, as a platform for screening potential promoters and inhibitors of neuroregeneration.

Tags

DrosophilaIn Vivo Injury ModelNeuroregenerationPeripheral Nervous SystemCentral Nervous SystemLive ImagingTwo-photon Laser Axotomy/dendriotomyGenetic ToolboxScreeningAxon RegenerationDendritic RegenerationNeurodegenerative DiseasesNeuron-glia InteractionsLarval CollectionCulture BottlesGrape Juice Agar PlateImagingTwo-photon MicroscopeGFPLaser IntensityPNS InjuryVNC Injury

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