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Metabolic Analysis of Drosophila melanogaster Larval and Adult Brains

Article DOI: 10.3791/58007-v • 07:06 min • August 7th, 2018
August 7th, 2018 •
1, 1, 1, 1, 1
1Department of Biology, Providence College

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We present a protocol for measuring oxygen consumption and extracellular acidification in Drosophila melanogaster larval and adult brains. A metabolic analyzer is utilized with an adapted and optimized protocol. Micro-tissue restraints are a critical component of this protocol and were designed and created specifically for their use in this analysis.

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Metabolic Analysis Drosophila Melanogaster Larval Brain Adult Brain Glial Cells Metabolic Reprogramming Diet Behavior Technique Intact Tissue Reproducible Results Micro-tissue Restraints Imaginal Discs C.elegans Mesh Basket Six-well Plate Ethanol De-ionized Water Assay Media Late-third Instar Larvae Dissecting Spot Plate
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