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Unraveling Key Players of Humoral Immunity
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Unraveling Key Players of Humoral Immunity: Advanced and Optimized Lymphocyte Isolation Protocol from Murine Peyer's Patches

Article DOI: 10.3791/58490-v • 08:25 min • November 21st, 2018
November 21st, 2018 •
1,2, 1,2, 1,2, 1,2, 1,2
1Division of Hematology-Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School

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In this study, we present a novel and effective protocol for the isolation of lymphocytes from Peyer's Patches (PPs), which can be subsequently used for in vivo and in vitro functional assays as well as flow cytometric studies of follicular T helper and germinal center B cells.

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Humoral Immunity Lymphocyte Isolation Protocol Murine Peyer's Patches T Cell Subsets B Cell Subsets Follicular T Helper Cells Germinal Center B Cells Lymphocyte Isolation Technique Collagenase Digestion Mouse Abdomen Sterilization Midline Abdominal Incision Peritoneal Cavity Cecum And Ileocecal Junction Small Intestine Detachment 6-well Plate RPMI Medium Fetal Bovine Serum (FBS) Mesenteric Fat
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