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Assay for Blood-brain Barrier Integrity in Drosophila melanogaster
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Assay for Blood-brain Barrier Integrity in Drosophila melanogaster

Article DOI: 10.3791/60233-v • 09:08 min • September 18th, 2019
September 18th, 2019 •
1, 1, 1
1Department of Biology, Loyola University Chicago

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Blood-brain barrier integrity is critical for nervous system function. In Drosophila melanogaster, the blood-brain barrier is formed by glial cells during late embryogenesis. This protocol describes methods to assay for blood-brain barrier formation and maintenance in D. melanogaster embryos and third instar larvae.

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Assay Blood-brain Barrier Integrity Drosophila Melanogaster Genetic Regulation Formation Maintenance Drosophila Development Permeability Intestinal Epithelium Organisms Technique Evaluation Live Tissues Visual Demonstration Sample Manipulations Embryo Collection Virgin Females Males Corn Meal Agar Food Incubate Flies Apple Juice Agar Plates Carbon Dioxide Anesthetized Flies Collection Cage Yeast Paste Red Sleeve
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