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Syngeneic Mouse Orthotopic Allografts to Model Pancreatic Cancer

Article DOI: 10.3791/64253-v • 06:20 min • October 4th, 2022
October 4th, 2022 •
1,2,3, 1,2,3,4, *1,2,3, *1,2,3
1Division of Translational Cancer Research, German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), 2Translational Cancer Research and Institute of Experimental Cancer Therapy, Klinikum rechts der Isar, School of Medicine, Technical University of Munich, 3Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, 4Department of Internal Medicine II, Klinikum rechts der Isar, Technical University of Munich
* These authors contributed equally

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Syngeneic mouse orthotopic allografts of pancreatic ductal adenocarcinoma (PDAC) recapitulate the biology, phenotypes, and therapeutic responses of disease subtypes. Owing to their fast, reproducible tumor progression, they are widely used in preclinical studies. Here, we show common practices to generate these models, injecting syngeneic murine PDAC cultures into the pancreas.

Tags

Syngeneic Mouse Orthotopic Allografts Pancreatic Cancer In Vivo Models Genotype Phenotype Drug Response Studies Tumor Progression Tumor Microenvironment Reproducibility Experiment Tumor Cells PDAC Cells Trypsin DMEM Fetal Bovine Serum Penicillin Streptomycin Centrifuge Cell Suspension Neubauer Chamber Cell Concentration Implantation
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