Tissue Biopsy
Tissue Biopsy - Related Videos
06:33
Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy
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Cited by 132 •
2015
We describe a method to establish human enteroids from small intestinal crypts and colonoids from colon crypts collected from both surgical tissue and biopsies. In this methodological article, we present the culture modalities that are essential for the successful growth and maintenance of human enteroids and colonoids.
09:01
A Technique for Subcutaneous Abdominal Adipose Tissue Biopsy via a Non-diathermy Method
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Cited by 7 •
2017
We standardized an abdominal adipose tissue biopsy using a non-diathermy method performed under local anesthesia. Three cases of excessive post-operation bleeding out of 115 operations (2.61%) occurred.We conclude that an abdominal adipose tissue surgical biopsy using a non-diathermy method can be safely applied to healthy men.
02:47
In Vitro Parallel-Plate Flow Model to Study Bacterial Adhesion on a Tissue Biopsy
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2026
Source: Ditkowski, B., et al. An In Vitro Model of a Parallel-Plate Perfusion System to Study Bacterial Adherence to Graft Tissues. J. Vis. Exp. (2019).This video demonstrates the method of using a parallel-plate flow chamber to perfuse fluorescently labeled bacteria over vascular graft tissues, enabling controlled analysis of bacterial adhesion and early pathogenesis under shear flow conditions.
11:27
Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy
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Cited by 24 •
2015
Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.
03:39
Colonoscopic Guided Pinch Biopsy in a Mouse Model: A Technique to Visualize and Extract Tissue Samples from Mouse Colon
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2025
This video demonstrates the protocol for performing colonoscope-guided pinch biopsy in the mouse. This technique helps visually analyze the colon lumen and collect tissue samples for detailed studies.
FAQs
Imaging shows anatomical patterns, but it may not establish what is causing an abnormality. Microscopic examination allows pathologists to evaluate tissue architecture and cellular changes directly, while molecular analysis can add information about disease-associated features. This combination helps distinguish processes such as inflammation, infection, abnormal cell growth, and cancer when clinical findings remain inconclusive.
Microscopy examines the structure and appearance of cells within preserved tissue, revealing patterns that support disease recognition. Molecular testing evaluates biological features that may not be visible under the microscope. Used together, these approaches can strengthen diagnostic interpretation, support tumor classification, and provide information relevant to selecting treatment or estimating prognosis.
Immunohistochemistry uses tissue analysis to assess selected biological markers, adding diagnostic detail beyond ordinary structural examination. Genomic testing examines molecular characteristics that can further distinguish disease subtypes or identify clinically relevant features. These methods extend the value of a biopsy from identifying abnormal tissue to supporting more precise diagnosis and personalized medicine.
The collection approach depends on the tissue site and the information clinicians need. A needle may obtain tissue through a focused sampling procedure, an endoscope may access tissue through an internal passage, and a surgical incision may be used when broader or more direct access is required. The selected approach determines how tissue reaches pathology for examination.
After collection, the sample is preserved so its features remain suitable for examination. Pathology processing then involves sectioning the tissue into thin portions and applying stains that make relevant structures easier to evaluate microscopically. Depending on the clinical question, the prepared material may also undergo immunohistochemistry or genomic testing, producing complementary structural and molecular findings.
Biopsy findings become especially valuable when symptoms or imaging do not provide a definitive diagnosis. Results can identify inflammation, infection, abnormal cell growth, or cancer, while additional analysis may classify a tumor more precisely. These findings can influence treatment selection and prognosis, making the procedure relevant not only to diagnosis but also to subsequent clinical planning.