Source: Koya, Y. et al. Murine Experimental Model of Original Tumor Development and Peritoneal Metastasis via Orthotopic Inoculation with Ovarian Carcinoma Cells. J. Vis. Exp. (2016)
This video describes the protocol for creating a murine experimental model to study tumor development and peritoneal metastasis. This ovarian cancer model is generated by the orthotopic inoculation of human epithelial ovarian cancer cells into the ovaries of a female mouse.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Preparation of Cell Suspensions
2. Orthotopic Inoculation with Cell Suspensions
NOTE: Surgery does not require a dedicated suite. Surgery can be performed on a laboratory benchtop in an area of the room that is separate and has minimal activity. A laminar hood also may be used. Sterile surgical instruments must be used, and scissors should not be used to make skin incisions.
The authors have nothing to disclose.
Matrigel matrix | BD | 354234 | ECM-based hydrogel |
Insulin syringe | TERUMO | SS-05M2913 | 1/2 cc, 29 G x 1/2" |
Suturing needle with suture | 11 mm, 3/8, Nylon6-0 | ||
Reflex 7 mm Wound Clip Applier | CellPoint Scientific | 204-1000 | |
Reflex 7 mm wound clips | CellPoint Scientific | 203-1000 |