Prepared by drawing capillary tubing into a fine 3-8 um with a shaft of approximately 100-300 um micropipette puller. When the needle is new, the tip is sealed and usually breaks in the first or second injection or the glass needle tip can be beveled using a Brown type micro-pipette beveller (Sutter Instrument).
Aedes aegypti
Animal
Male and female mosquitos, to obtain embryos.
Drosophila culture vial
For mosquito egg-laying.
Halocarbon oil
Reagent
Oil for desiccation: Halocarbon oil 700:Halocarbon oil 27(1:1)
Jasinskiene, N., Juhn, J., James, A. A. Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes. J. Vis. Exp. (5), e219, doi:10.3791/219 (2007).