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Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy
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Bioengineering
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JoVE Journal Bioengineering
Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy

Spatio-Temporal In Vivo Imaging of Ocular Drug Delivery Systems using Fiberoptic Confocal Laser Microendoscopy

DOI:
10.3791/62685-v

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07:12 min

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September 27, 2021

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, , ,
1Laboratory for Translational and Molecular Imaging, Cancer and Stem Cell Biology Programme,Duke-NUS Medical School, 2School of Materials Science and Engineering,Nanyang Technological University, 3Singapore National Eye Centre, 4Singapore Eye Research Institute, 5Material Science & Engineering,National University of Singapore

Chapters

  • 00:05Introduction
  • 00:54Evans Blue and Liposomes Administration in Live Mouse
  • 02:00Confocal Laser Microscopy Set-up
  • 04:28Live Imaging with CLM and Acquisition
  • 05:00Results: Subconjunctival Liposome Injection and In Vivo Imaging Using Fiberoptic Confocal Laser Microendoscopy
  • 06:29Conclusion

Summary

Automatic Translation

Automatic Translation

We present a protocol for the use of fiberoptic confocal laser microendoscopy (CLM) to non-invasively study the spatio-temporal distribution of liposomes in the eye after subconjunctival injection.

Tags

Keywords: Spatio-temporal ImagingOcular Drug DeliveryFiberoptic Confocal Laser MicroendoscopyIn Vivo ImagingFluorescent LiposomesEvans BlueSubconjunctival InjectionReal-time ImagingNoninvasiveAnimal Reduction

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