Cellular Fibronectin
Cellular Fibronectin - Related Videos
10:57
Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
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Cited by 2 •
2019
This method is useful for quantifying the early dynamics of cellular adhesion and spreading of anchorage-dependent cells onto the fibronectin. Furthermore, this assay can be used to investigate the effects of altered redox homeostasis on cell spreading and/or cell adhesion-related intracellular signaling pathways.
01:25
Fibronectins Connect Cells with ECM
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2023
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal. Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
08:09
Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay
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Cited by 5 •
2024
This protocol details the isolation of chondrocytes, Fibronectin Adhesion Assay-derived Chondroprogenitors (FAA-CPs), and Migratory Chondroprogenitors (MCPs) from human articular cartilage. It covers enzymatic digestion, fibronectin adhesion, and migration-based assays for isolating and characterizing these cells.
11:38
Preparation of Complaint Matrices for Quantifying Cellular Contraction
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Cited by 115 •
2010
In this video, we demonstrate the experimental techniques used to fabricate compliant, extracellular matrix (ECM) coated substrates suitable for cell culture, and which are amenable to traction force microscopy and observing effects of ECM stiffness on cell behavior.
05:00
Cellular Respiration - Concepts
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2019
Autotrophs and Heterotrophs Living organisms require a continuous input of energy to maintain cellular and organismal functions such as growth, repair, movement, defense, and reproduction. Cells can only use chemical energy to fuel their functions, therefore they need to harvest energy from chemical bonds of biomolecules, such as sugars and lipids. Autotrophic organisms, namely plants, algae, and photosynthetic and chemosynthetic bacteria, convert inorganic materials into such biomolecules by...
FAQs
Cells first bind secreted fibronectin through integrins, cell-surface adhesion receptors. Cytoskeletal tension then acts on these attachments, allowing cells to organize the protein into fibrillar networks. This mechanically coupled assembly gives the extracellular matrix an organized structure that can influence how cells attach, move, and communicate.
Alternative splicing generates cellular fibronectin variants with functions distinct from plasma fibronectin. This means fibronectin should not be treated as a single uniform molecular form when interpreting extracellular matrix biology. Comparing variants can help relate molecular composition to differences in tissue organization, cell behavior, or communication during development, repair, and remodeling.
The fibrillar network does more than provide physical support. Its organization creates an extracellular context that regulates cell migration, proliferation, and differentiation. Because these behaviors are central to tissue organization and remodeling, changes in how cells assemble or arrange fibronectin can alter cell responses even when the protein is present.
During embryonic development and wound repair, cellular fibronectin networks help coordinate the extracellular environment in which cells organize and respond. Their effects on migration, proliferation, and differentiation connect matrix assembly with the changing demands of growing or repairing tissue. For this reason, studies of these settings use fibronectin to examine how cell behavior contributes to tissue formation and restoration.
Abnormal production or organization of cellular fibronectin can reflect disruption in normal extracellular matrix regulation. In fibrosis, tumor progression, and other diseases, examining these changes helps place altered cell adhesion, communication, migration, proliferation, or differentiation within a broader tissue context. The problem may concern how much fibronectin cells produce, how they organize it, or both.
Cellular fibronectin is relevant to regenerative medicine because tissue restoration depends on coordinated cell behavior and organization. Its fibrillar networks provide a framework for studying how adhesion and communication relate to migration, proliferation, and differentiation during repair. This connection helps researchers link extracellular matrix biology with tissue remodeling and with strategies intended to understand or support regeneration.