Colorectal Cancer
Colorectal Cancer - Related Videos
03:19
Generating PDOX Mouse Model of Colorectal Cancer: Studying Progression of Colorectal Cancer in a Murine Model
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2023
This video describes the protocol for generating a patient-derived orthotopic xenograft colorectal cancer mouse model to study the progression of colorectal cancer cells in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. This murine model helps in understanding primary tumor growth and metastases pattern.
08:43
Orthotopic Mouse Model of Colorectal Cancer
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Cited by 109 •
2007
Two techniques can be used to establish this model: injection of a cancer cell suspension into the cecal wall or transplantation of a piece of subcutaneous tumor onto the cecum. This model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.
06:01
A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
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Cited by 3 •
2017
A protocol for the establishment of a genetically engineered mouse model of colorectal cancer by segmental adeno-cre infection and its surveillance via high-resolution colonoscopy is presented.
06:19
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
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Cited by 13 •
2017
We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.
03:41
CRC Organoid Culture: A Method to Obtain 3D Organoids from Colorectal Cancer Cells
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2023
This video describes the detailed protocol for generating 3D organoids from murine-model derived colorectal cancer cells by propagating them on artificial extracellular matrix coated plates. These CRC organoids can be used as a model to study the primary growth of human tumor cells and metastasis.
FAQs
Accumulated genetic and epigenetic alterations can disrupt pathways that regulate cell growth, DNA repair, and apoptosis, the programmed removal of damaged cells. When these controls become abnormal, cells may survive, divide, and acquire additional changes over time. Studying these alterations helps biology researchers explain tumor development and identify molecular features that may guide classification or treatment decisions.
These factors can affect whether abnormal cellular changes arise, persist, or progress. Inherited risk may increase susceptibility, while environmental influences, inflammation, and interactions with the gut microbiome may shape the conditions surrounding intestinal cells. Research examines how these influences combine rather than treating tumor development as the result of a single cause.
Tumor behavior depends not only on changes within abnormal cells but also on their interactions with nearby tissue. These relationships can influence tumor progression and the ability of cancer to spread to other tissues. Investigating the surrounding environment therefore adds biological context to studies of growth, disease advancement, and variation in treatment response.
Molecular classification organizes tumors according to biologically relevant features, including changes affecting growth control, DNA repair, apoptosis, or tissue interactions. This approach can reveal that tumors with the same anatomical location may differ in underlying mechanisms. Such distinctions support more precise research into prognosis, treatment selection, and why individual tumors respond differently.
Screening supports the identification of disease-related changes before or during clinically important progression, although the overview does not specify particular screening tests or schedules. In biology and medicine, screening findings can be examined alongside inherited, molecular, inflammatory, and microbiome-related factors. This connection helps researchers investigate earlier intervention and prevention strategies.
Targeted therapies and immunotherapies apply biological knowledge about tumor mechanisms and interactions with the surrounding tissue. Molecular findings can indicate which growth or repair pathways require closer study, while immune-focused approaches reflect the importance of tumor-host interactions. Research also evaluates treatment response, helping clarify why therapeutic effects vary among tumors and patients.