Embryonic Skin Culture
Embryonic Skin Culture - Related Videos
08:29
Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration
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Cited by 11 •
2014
We describe the dissection and ex vivo culture of mouse embryonic skin. The culture system maintains an air-liquid interface across the tissue surface and allows imaging on an inverted microscope. Melanoblasts, a component of the developing skin, are fluorescently labeled allowing their behavior to be observed using confocal microscopy.
10:51
A System for ex vivo Culturing of Embryonic Pancreas
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Cited by 23 •
2012
Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.
09:52
Embryonic Stem Cell Culture and Differentiation
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2023
Culturing embryonic stem (ES) cells requires conditions that maintain these cells in an undifferentiated state to preserve their capacity for self-renewal and pluripotency. Stem cell biologists are continuously optimizing methods to improve the efficiency of ES cell culture, and are simultaneously trying to direct the differentiation of ES cells into specific cell types that could be used in regenerative medicine. This video describes the basic principles of ES cell culture, and demonstrates a...
09:10
A Method for Culturing Embryonic C. elegans Cells
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Cited by 13 •
2013
We describe here a revised protocol for large-scale culture of embryonic C. elegans cells. Embryonic C. elegans cells cultured in vitro using this method, appear to differentiate and recapitulate the expression of genes in a cell specific manner. Techniques that require direct access to the cells or isolation of specific cell types from the other tissues can be applied on C. elegans cultured cells.
07:23
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
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Cited by 24 •
2016
We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.
FAQs
The epidermis and dermis remain informative because their interaction can continue after dissection. Maintaining the tissue in nutrient medium preserves key cellular behaviors and developmental signaling, allowing investigators to examine how signals between these layers contribute to organization and maturation. This layered relationship is central to interpreting changes observed during culture.
Controlled laboratory conditions make it possible to alter the experimental environment and observe developmental responses directly. Because the tissue remains outside the organism, researchers can connect a manipulated condition with changes in cellular behavior, tissue organization, or signaling more readily than through observation alone. This makes the model useful for testing mechanisms of skin development.
Observations can include epidermal differentiation, hair follicle and other appendage formation, and tissue organization. Examining these processes together helps link cell maturation with larger-scale patterning rather than treating the epidermis as an isolated layer. The model therefore provides a way to study coordinated organ development while retaining interactions between skin compartments.
A typical workflow begins when embryonic skin is dissected and placed in nutrient medium under controlled laboratory conditions. The cultured tissue is then observed for continued cellular behavior, developmental signaling, organization, and maturation. Experimental manipulation can be introduced to investigate specific responses, making dissection, maintenance, observation, and manipulation the central stages.
By retaining developing tissue interactions, the culture can support direct study of wound repair alongside normal skin maturation. Researchers can examine how repair relates to epidermal and dermal behavior and tissue organization. These observations make the model relevant to regenerative biology, while experimental manipulation can help investigate developmental processes associated with congenital skin disorders.
Appendage formation can be examined within the developing skin rather than as an isolated event. In culture, researchers can observe hair follicle and other appendage development together with epidermal differentiation, dermal interactions, and tissue organization. This combined view helps connect visible structural outcomes with the broader developmental behavior of the skin as an organ.