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JoVE Journal
Developmental Biology

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Culture of Murine Embryonic Metatarsals
 

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Article DOI: 10.3791/54978
December 3rd, 2016

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Summary December 3rd, 2016

We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.

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