Epidermal Growth Factor Removal
Epidermal Growth Factor Removal - Related Videos
09:16
Studying the Stoichiometry of Epidermal Growth Factor Receptor in Intact Cells using Correlative Microscopy
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Cited by 23 •
2015
This protocol describes the labeling of epidermal growth factor receptor (EGFR) on COS7 fibroblast cells, and subsequent correlative light- and electron microscopy of whole cells in hydrated state. The label contained fluorescent quantum dots. The protocol can be used to study the stoichiometry of EGFR at the single molecule level.
06:41
Removal of Drosophila Muscle Tissue from Larval Fillets for Immunofluorescence Analysis of Sensory Neurons and Epidermal Cells
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Cited by 13 •
2016
Studies of neuronal morphogenesis using Drosophila larval dendritic arborization (da) neurons benefit from in situ visualization of neuronal and epidermal proteins by immunofluorescence. We describe a procedure that improves immunofluorescence analysis of da neurons and surrounding epidermal cells by removing muscle tissue from the larval body wall.
18:27
Preparing T Cell Growth Factor from Rat Splenocytes
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Cited by 12 •
2007
We describe the preparation of T cell growth factor used for the in vitro expansion of antigen-specific rat T lymphocyte lines.
09:04
A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors
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Cited by 7 •
2016
We describe a simple cell-based bioassay for detecting, quantifying and monitoring the activity of members of the vascular endothelial growth factor family of ligands. The assay uses chimeric receptors expressed in a factor-dependent cell line to provide a semi-quantitative or quantitative assessment of receptor binding and cross-linking by the ligand.
08:28
Validated Immunochemical Assay for Comprehensive Determination of the Human Epidermal Growth Factor Receptor 2 Released from and Bound to Cells
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2025
We present a validated sandwich ELISA assay using novel anti-HER2 monoclonal antibodies. This assay enables precise quantification of cell-bound and released HER2 protein from in vitro cultured cells and other samples, including blood and tissues.
FAQs
Removing EGF reduces stimulation of the epidermal growth factor receptor, or EGFR, which lowers activity in downstream signaling pathways. Because these pathways help regulate proliferation, survival, and cellular state, withdrawal allows researchers to observe which cellular behaviors depend on continued EGF input. The response therefore connects changes in cell behavior with reduced growth-factor signaling.
EGF removal creates a controlled comparison for determining whether an observed cellular response requires EGF rather than another growth factor. This distinction helps researchers interpret changes in growth, survival, or cellular state more precisely. It is especially useful in systems where several growth-factor signals operate simultaneously and may produce overlapping effects on the same cells.
Responses vary because cell systems differ in how strongly their behavior depends on EGF-mediated signaling. Withdrawal may produce measurable changes in proliferation, survival, or cellular state in one model, while another may show a different pattern. Comparing stem cells, organoids, and tissue models can therefore reveal how cellular context shapes growth-factor requirements and adaptation.
The central step is to replace medium containing EGF with medium lacking EGF, thereby withdrawing the specified signal from the biological system. Researchers then examine the resulting cellular response, such as altered growth or state. This approach is designed to change EGF availability while providing a controlled way to study the consequences of reduced receptor pathway activation.
Researchers can assess changes in cell proliferation, survival, and cellular state after EGF is removed. These outcomes indicate how strongly the system relies on EGF-dependent signaling and whether cells adapt when that input is reduced. Measurable changes can help identify growth-factor requirements and clarify how signaling conditions influence the behavior of cultured biological models.
EGF removal supports investigations in stem-cell systems, organoids, and tissue models, as well as broader studies of receptor signaling. In these settings, the method helps test whether EGF is required to maintain a particular growth or cellular state. It also provides context for studying how biological systems respond when a defined growth-factor input is withdrawn.