Flow Through Assembly
Flow Through Assembly - Related Videos
11:36
Assembly, Loading, and Alignment of an Analytical Ultracentrifuge Sample Cell
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Cited by 21 •
2009
The analytical ultracentrifuge (AUC) sample cell holds sample and reference buffer and during experiments and is exposed to high vacuum and rotor speeds up to 60,000 rpm. This video will demonstrate the rigorous attention to detail necessary for assembly, loading and alignment of this very important component of an AUC experiment.
05:58
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
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Cited by 16 •
2019
This article describes a FRET-based flow cytometry protocol to quantify protein self-assembly in both S. cerevisiae and HEK293T cells.
09:20
The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
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Cited by 10 •
2016
Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.
08:32
Assembly and Characterization of an External Driver for the Generation of Sub-Kilohertz Oscillatory Flow in Microchannels
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2022
The protocol demonstrates a convenient method to produce harmonic oscillatory flow from 10-1000 Hz in microchannels. This is performed by interfacing a computer-controlled speaker diaphragm to the microchannel in a modular manner.
13:05
Micropatterning and Assembly of 3D Microvessels
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Cited by 12 •
2016
This manuscript presents an injection molding method to engineer microvessels that recapitulate physiological properties of endothelium. The microfluidic-based process creates patent 3D vascular networks with tailorable conditions, such as flow, cellular composition, geometry, and biochemical gradients. The fabrication process and examples of potential applications are described.
FAQs
Residence time determines how long components remain within the controlled fluidic system before moving onward. Together with fluid movement, it affects the opportunity for reactants to contact one another, bind, and organize. Adjusting this timing can therefore influence assembly behavior and reproducibility, making residence time an important variable when researchers define operating conditions.
Sequential contact allows reactants to meet in a planned order rather than all at once. This arrangement can coordinate binding or organization as components move through different stages of the system. Controlled concentration and mixing at each contact point help researchers guide assembly under defined conditions and examine how particular interactions contribute to the resulting biological structure.
A static vessel holds reacting components in one location, whereas Flow Through Assembly links component movement with controlled fluidic conditions. The flow-through approach can regulate contact, concentration, mixing, and residence time as material progresses through the system. This distinction supports continuous processing, automation, and more consistent control of assembly conditions than a single stationary reaction environment.
Reproducibility depends on controlling how components are mixed, when they contact one another, and the concentrations present during each stage. Residence time also needs definition because it determines exposure to the relevant conditions. Managing these factors within the fluidic system helps produce more consistent binding, organization, or assembly across repeated runs.
A typical workflow introduces biological or biomolecular components into a controlled fluidic system, brings them into sequential contact, and maintains defined mixing and concentration conditions as they move through the system. Residence time is controlled during this progression, allowing assembly to occur before the resulting material exits or advances to a later stage. The linked process can also be automated.
Researchers may choose Flow Through Assembly when they need reproducible construction of molecular complexes, engineered biomaterials, or other organized biological systems. It is especially relevant when continuous processing, automation, or high-throughput operation is useful. By connecting fluid control with assembly behavior, the method also provides a way to study how biological structures form under defined conditions.