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Chapters
- 00:05Title
- 02:08Library Preparation: End Repair
- 04:12Library Preparation: dA-Tailing and Adapter Ligation
- 05:55Roche Nimblegen SeqCAP EZ Library Hybridization, Wash, and Amplification
- 08:31Tumor-normal Pair Analysis
- 10:08Conclusion
We describe the preparation of barcoded DNA libraries and subsequent hybridization-based exon capture for detection of key cancer-associated mutations in clinical tumor specimens by massively parallel "next generation" sequencing. Targeted exon sequencing offers the benefits of high throughput, low cost, and deep sequence coverage, thus yielding high sensitivity for detecting low frequency mutations.
