Genome Engineering
Genome Engineering - Related Videos
12:04
Mouse Genome Engineering Using Designer Nucleases
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Cited by 14 •
2014
Designer nucleases such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can be used to modify the genome of mouse preimplantation embryos by triggering both the nonhomologous end joining (NHEJ) and homologous recombination (HR) pathways. These advances enable the rapid generation of mice with precise genetic modifications.
08:20
Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
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Cited by 13 •
2020
Here we provide a detailed, step-by-step protocol for CRISPR/Cas9-based genome engineering of primary human B cells for gene knockout (KO) and knock-in (KI) to study biological functions of genes in B cells and the development of B-cell therapeutics.
08:31
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
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Cited by 2 •
2013
Single Virus Genomics (SVG) is a method to isolate and amplify the genomes of single virons. Viral suspensions of a mixed assemblage are sorted using flow cytometry onto a microscope slide with discrete wells containing agarose, thereby capturing the virion and reducing genome shearing during downstream processing. Whole genome amplification is achieved using multiple displacement amplification (MDA) resulting in genomic material that is suitable for sequencing.
05:01
Agrobacterium-Mediated Genetic Transformation: A Method to Genetically Transform the Rice Genome via Genetically Engineered Agrobacterium tumefaciens
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2025
In this video, we demonstrate genetic transformation of the rice genome using an Agrobacterium tumefaciens binary vector. This bacterium facilitates the transfer of a foreign DNA plasmid using Agrobacterium virulence proteins.
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
FAQs
After a targeted DNA break or rearrangement, the cell’s own repair pathways help determine what sequence change results. Repair can produce insertions, deletions, or substitutions, while a supplied donor-derived sequence can become incorporated into the genome. Consequently, the same targeting strategy may support different experimental goals depending on whether researchers need to disrupt, replace, or introduce genetic material.
These tools provide different ways to direct genetic change. CRISPR-Cas systems and engineered nucleases can create targeted DNA breaks, whereas recombinases support specific DNA rearrangements. Their shared value is controlled intervention at selected genetic sites, but the type of tool influences whether an experiment emphasizes cutting, rearrangement, or the introduction of a defined sequence.
Targeting accuracy helps researchers modify the intended genetic location, while control over editing outcomes helps them obtain the desired insertion, deletion, substitution, or donor-derived sequence. These factors directly affect how confidently a result can be linked to a gene or genetic change. Improving both expands genome engineering from exploratory experiments toward more controlled biological and therapeutic development.
Genome engineering can test gene function by changing the underlying genetic material rather than only observing gene activity or association. Researchers may create insertions, deletions, substitutions, or rearrangements and then examine the resulting biological effects. This makes the approach useful for connecting a specific genetic alteration with function, disease-related biology, or a useful biological trait.
A typical workflow begins by selecting a genetic target and an appropriate tool, such as a CRISPR-Cas system, engineered nuclease, or recombinase. The intervention then creates a targeted DNA break or rearrangement, with cellular repair producing the intended type of change. Researchers subsequently examine the resulting genetic material and biological effects to assess the experimental outcome.
Applications span functional genomics, genetic disease research, drug development, crop improvement, and the design of engineered cells. In functional genomics, altered DNA helps investigate gene roles; in disease research, it supports genetic disease models. The same general approach can also help develop useful traits, evaluate biological targets, and build cells designed for particular research or biotechnology purposes.
The method can reveal how a gene contributes to a biological process, how a genetic change relates to disease, or whether a specific alteration produces a useful trait. By connecting controlled DNA changes with observed outcomes, researchers can generate evidence for gene function and disease mechanisms. These findings can also inform drug development and the design of engineered cells.