High Throughput Data

High-throughput data are large, rapidly generated datasets produced by parallelized experiments, automated instruments, or computational analyses, enabling biology to measure many genes, cells, molecules, or conditions at once. In biological research, platforms such as next-generation sequencing, mass spectrometry, and microarrays create millions of measurements that require quality control, normalization, and statistical processing before interpretation. These data support genomics, transcriptomics, proteomics, drug screening, and systems biology by revealing patterns that single-measurement approaches can miss. Reproducible computational workflows help researchers identify disease-associated changes, infer biological networks, and prioritize targets for further study.

High Throughput Data - Related Videos

Research

JoVE Journal - Biology

High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes

0 Views •

2015

Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.

Research

JoVE Journal - Medicine
Free Sample

High-Throughput Analysis of Optical Mapping Data Using ElectroMap

0 Views •

Cited by 13 •

2019

This protocol describes the setup and use of ElectroMap, a MATLAB-based open-source software platform for analysis of cardiac optical mapping data. ElectroMap provides a versatile high-throughput tool for analysis of optical mapping voltage and calcium datasets across a wide range of cardiac experimental models.

Research

JoVE Journal - Biochemistry
Free Sample

High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

0 Views •

Cited by 16 •

2023

This protocol details high-throughput crystallization screening, ranging from the 1,536 microassay plate preparation to the end of a 6 week experimental time window. Details are included about the sample setup, the imaging obtained, and how users can perform analyses using an artificial intelligence-enabled graphical user interface to quickly and efficiently identify macromolecular crystallization conditions.

Designing a Bioreactor to Improve Data Acquisition and Model Throughput of Engineered Cardiac Tissues

0 Views •

Cited by 2 •

2023

Three-dimensional cardiac tissues bioengineered using stem-cell-derived cardiomyocytes have emerged as promising models for studying healthy and diseased human myocardium in vitro while recapitulating key aspects of the native cardiac niche. This manuscript describes a protocol for fabricating and analyzing high-content engineered cardiac tissues generated from human induced pluripotent stem-cell-derived cardiomyocytes.

A Semi-high-throughput Imaging Method and Data Visualization Toolkit to Analyze C. elegans Embryonic Development

0 Views •

Cited by 2 •

2019

This work describes a semi-high-throughput protocol that allows simultaneous 3D time-lapse imaging of embryogenesis in 80–100 C. elegans embryos in a single overnight run. Additionally, image processing and visualization tools are included to streamline data analysis. The combination of these methods with custom reporter strains enables detailed monitoring of embryogenesis.

View All Results

FAQs

Related Topics