Automated Sample Preparation for the Multiplexed Analysis of Single-Cell Histone Post-Translational Modifications (sc-hPTM2)

Here, we present a protocol to automate single-cell histone post-translational modification analysis using an isotopic two-plex labeling strategy and ArgC digestion. This workflow enables quantitative, reproducible, and high-sensitivity profiling of chromatin heterogeneity and epigenetic responses at single-cell resolution.