Automated Sample Preparation for the Multiplexed Analysis of Single-Cell Histone Post-Translational Modifications (sc-hPTM2)

We're advancing single cell proteomics with an automated multiplex method to study global chromatin heterogeneity for post-translational modifications. What makes this protocol challenging is the picogram scale proteomic preparation, multiplex labeling, as well as resolving complex combinatorial modified histone peptides. To begin, reconstitute Hep G2/C3A hepatocellular carcinoma cells at a final concentration of 200 to 500 cells per microliter in ice cold PBS.

Set each nozzles pulse width and drive voltage to the values provided by the manufacturer. For maximal pickup efficiency, ensure that the Z offset between the two nozzles differs by less than 50 micrometers. Identify the optimal contact position for each nozzle and compare the Z height readouts in the nozzle setup tab.