Luciferin-luciferase Reagent
Luciferin-luciferase Reagent - Related Videos
08:09
High-throughput Titration of Luciferase-expressing Recombinant Viruses
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Cited by 14 •
2014
This article presents a high-throughput luciferase expression-based method of titrating various RNA and DNA viruses using automated and manual liquid handlers.
03:34
Luciferase-Based Growth Assay to Quantify Intracellular Parasite Reproduction
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2025
In this video, we demonstrate the luciferase assay to quantify the growth of the intracellular pathogen Toxoplasma gondii. Utilizing a strain of Toxoplasma that expresses luciferase enzyme after infecting the host cells, the luciferase activity is detected in the infected cell culture to determine the successful reproduction of the parasite.
03:49
A Luciferase-Fluorescent Reporter Influenza Virus to Track Viral Infections
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2025
This video demonstrates a method to track viral infections in mouse models using a recombinant luciferase and fluorescence protein-expressing bi-reporter Influenza A virus. This method enables both in vivo and ex vivo studies, enabling researchers to gain insights into viral dynamics and the effects of interventions.
03:24
ATP-Based Luciferase Viability Assay: A Homogenous Method to Evaluate the Growth-Inhibitory Potential of Test Agents on Tumor Organoids
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2025
In this video, we demonstrate a rapid, single-addition assay to assess cell viability in patient-derived organoids upon treatment with a test agent using intracellular ATP levels as a marker for cellular metabolic activity. The intracellular ATP levels are measured using the luciferase/luciferin reaction, where the emitted light intensity indicates the cellular viability and, in turn, the test agent's growth-inhibitory potency.
12:58
Transfecting RAW264.7 Cells with a Luciferase Reporter Gene
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Cited by 13 •
2015
Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.
FAQs
Cofactors create the conditions required for luciferase to oxidize luciferin efficiently and convert chemical energy into emitted light. Changes in their availability can therefore alter signal intensity independently of the amount of luciferase present. Controlling these reaction conditions is essential when comparing samples or interpreting luminescence as a quantitative biological readout.
The measured signal reflects two linked factors: the reaction conditions that permit light production and the amount of reporter enzyme available. Consequently, a stronger or weaker signal does not automatically indicate a change in the biological process being studied. Experimental interpretation requires attention to both reagent performance and reporter abundance.
Unlike measurements that require excitation illumination, this system generates light through the reaction itself. The detector therefore records emitted bioluminescence without an excitation-light step. That feature supports sensitive quantitative measurements and is particularly useful when researchers need to follow reporter activity, cellular energy status, or biological processes over time.
A basic measurement workflow combines the reagent with a biological sample under appropriate cofactor conditions, allows the enzyme-catalyzed reaction to generate light, and records the emitted signal. Researchers then relate the measured intensity to the experimental question, such as reporter-enzyme availability, ATP-associated cellular energy status, or enzyme activity.
In reporter gene assays, the reagent converts the presence of a luciferase-linked reporter enzyme into a measurable optical signal. This enables quantitative analysis of gene expression because changes in reporter availability can alter emitted light. The approach provides a biochemical readout that can be compared across experimental conditions to assess differences in expression.
Applications include ATP measurements, enzyme studies, reporter-based analysis of gene expression, and imaging in living cells or organisms. These uses connect emitted light with cellular energy status, enzyme activity, or reporter availability. Because measurements can be collected over time, the system also helps researchers examine changing biological processes rather than only a single endpoint.