Mouse Mammary Model
Mouse Mammary Model - Related Videos
08:51
Evaluation of Mammary Gland Development and Function in Mouse Models
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Cited by 98 •
2011
This method describes how to dissect and assess mammary gland development and function from mice. Excised mammary glands are assessed for the degree of development using whole mount while milk ejection is evaluated using an oxytocin-based myoepithelial cell contraction assay.
06:39
Intraductal Injection for Localized Drug Delivery to the Mouse Mammary Gland
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Cited by 36 •
2013
A protocol for the non-invasive intraductal delivery of aqueous reagents to the mouse mammary gland is described. The method takes advantage of localized injection into the nipples of mammary glands targeting mammary ducts specifically. This technique is adaptable for a variety of compounds including siRNA, chemotherapeutic agents and small molecules.
11:13
Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland
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Cited by 19 •
2015
The indirect immunofluorescence protocol described in this article allows the detection and the localization of proteins in the mouse mammary gland. A complete method is given to prepare mammary gland samples, to perform immunohistochemistry, to image the tissue sections by fluorescence microscopy, and to reconstruct images.
11:47
Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
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Cited by 4 •
2013
Time-lapse imaging is used to assess behavior of primary preneoplastic mammary epithelial cells derived from genetically engineered mouse models of breast cancer risk to determine if there are correlations between specific behavioral parameters and distinct genetic lesions.
03:45
Intraductal Injection: Delivering Injection Mix into the Ducts of the Mouse Mammary Gland
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2023
This video describes an intraductal injection technique where we deliver injection mix into the ducts of mouse mammary glands.
FAQs
Hormonal signals and pregnancy-associated changes influence how mammary epithelial cells organize, expand, and remodel ducts and alveoli. Observing these responses in a living organism allows researchers to connect changing physiological conditions with tissue structure and function. This makes the model useful for examining developmental transitions and the biological processes that support lactation.
Mammary epithelial cells do not develop in isolation; their behavior is examined alongside interactions with the surrounding tissue. These relationships help determine how ducts and alveoli form and remodel. Studying both epithelial responses and tissue context can therefore reveal mechanisms of normal gland development that would be difficult to understand by analyzing epithelial cells alone.
Genetic manipulation enables controlled testing of how particular genes influence mammary development, cell behavior, and disease mechanisms. Tissue transplantation provides another way to examine mammary tissue in an experimental setting, while tissue analysis identifies resulting changes. Together, these approaches help separate gene-associated effects from broader changes observed during gland remodeling or disease progression.
Researchers combine genetic manipulation, tissue transplantation, and analysis of mammary tissue to study mammary biology. Genetic approaches test gene function, transplantation examines tissue behavior under controlled experimental conditions, and tissue analysis evaluates structural or biological changes. Using these approaches together supports investigations of development, stem and progenitor cell behavior, tumor initiation, and progression.
The model is especially valuable when researchers need to connect normal mammary biology with disease development in a living organism. It can be used to investigate tumor initiation and breast cancer progression while retaining mammary tissue interactions and physiological changes. This context supports controlled studies of disease mechanisms and the evaluation of potential therapeutic strategies.
By tracking mammary epithelial responses, tissue remodeling, and disease-associated changes, the system links basic developmental biology with applied cancer research. Studies can examine normal gland formation, lactation-related biology, stem and progenitor cell behavior, tumor initiation, or progression. The same experimental framework can also help evaluate therapeutic approaches in the context of mammary tissue.