Journal
/
Medicine
/
Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

Time-lapse Imaging of Primary Preneoplastic Mammary Epithelial Cells Derived from Genetically Engineered Mouse Models of Breast Cancer

DOI:
10.3791/50198-v

•

11:47 min

•

February 08, 2013

•

, , , , , , ,
1Department of Oncology,Georgetown University, 2Lombardi Comprehensive Cancer Center,Georgetown University, 3Stem Cell Dynamics,Helmholtz Zentrum München – German Research Center for Environmental Health, 4Department of Medicine,Georgetown University, 5Department of Nanobiomedical Science and WCU Research Center of Nanobiomedical Science,Dankook University

Chapters

  • 00:05Title
  • 01:48Generation of Primary Mammary Epithelial Cell Cultures
  • 03:39Live-cell Imaging
  • 05:56Assessing Cellular Event from Time-lapse Images
  • 07:50Making Single Cell Fate Maps with Timm’s Tracking Tool (TTT)
  • 09:59Representative Results: Colony Formation in BRCA1 Disrupted Cells
  • 11:12Conclusion

Summary

Automatic Translation

Automatic Translation

Time-lapse imaging is used to assess behavior of primary preneoplastic mammary epithelial cells derived from genetically engineered mouse models of breast cancer risk to determine if there are correlations between specific behavioral parameters and distinct genetic lesions.

Tags

Keywords: Time-lapse ImagingPrimary Preneoplastic Mammary Epithelial CellsGenetically Engineered Mouse ModelsBreast CancerCell DivisionApoptosisColony FormationCell MorphologySingle-cell TrackingCell Fate Maps

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/50198?article_type=v

In vivo Bioluminescence Imaging of Tumor Hypoxia Dynamics of Breast Cancer Brain Metastasis in a Mouse Model
check_url/50198?article_type=v

Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
check_url/50198?article_type=v

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer
check_url/50198?article_type=v

Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
check_url/50198?article_type=v

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
check_url/50198?article_type=v

Method for Obtaining Primary Ovarian Cancer Cells From Solid Specimens
check_url/50198?article_type=v

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.
check_url/50198?article_type=v

Tracking the Mammary Architectural Features and Detecting Breast Cancer with Magnetic Resonance Diffusion Tensor Imaging
check_url/50198?article_type=v

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes
check_url/50198?article_type=v

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

Read Article