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Chapters
- 00:05Title
- 00:45Exposure of Pre-filtration Sentinels Out of a Recirculating System
- 02:41Sump Swabs
- 03:42P. tomentosa Eggs Detection at the Bottom of a Tank and Animal Screening
- 06:48PCR on Sludge Sediment
- 08:12Results: Sump Swabs are Valuable Alternative to Identify Prevalent Mycobacterium
- 09:21Conclusion
This protocol describes the use of sump swabs and sludge analysis of zebrafish systems, which leads to increased detection compared to the sole use of sentinels to detect pathogens such as Aeromonas hydrophila, Mycobacterium spp., and Pseudocapillaria tomentosa. A system to monitor P. tomentosa eggs in quarantine is also proposed.
