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Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
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Medicine
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JoVE Journal Medicine
Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

DOI:
10.3791/52801-v

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07:29 min

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June 20, 2015

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, , , ,
1Stem Cells & Cancer Group, Molecular Pathology Program,Spanish National Cancer Research Center, 2Institute for Research in Biomedicine (IRB Barcelona), 3Center for Stem Cells in Cancer & Ageing, Barts Cancer Institute,Queen Mary University of London

Chapters

  • 00:05Title
  • 01:28Isolation of Cancer Stem Cells from Pancreatic Ductal Adenocarcinoma
  • 02:58Sphere Formation Assay and Metformin Treatment
  • 04:21Metabolism Analysis: ROS Production and Oxygen Consumption
  • 06:09Results: The Effects of Metformin on Pancreatic CSC Function
  • 06:59Conclusion

Summary

Automatic Translation

Automatic Translation

Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is provided.

Tags

Keywords: Pancreatic CancerCancer Stem CellsTumor SpheresMetforminReactive Oxygen SpeciesCancer Treatment Strategies

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