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Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

DOI:
10.3791/58678-v

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06:47 min

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November 26, 2018

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, , ,
1Department of Anatomy and Embryology,Leiden University Medical Center, 2Department of Applied Stem Cell Technologies,University of Twente

Chapters

  • 00:04Title
  • 00:36Microfluidic Chip Coating and Human Induced Pluripotent Stem Cell-derived Endothelial Cell (hiPSC-EC) Seeding
  • 02:17Live Imaging and Adherent Leukocyte Quantification
  • 05:21Results: Representative Adherent Leukocyte Identification
  • 06:09Conclusion

Summary

Automatic Translation

Automatic Translation

This step-by-step protocol provides a detailed description of the experimental setup and data analysis for the assessment of inflammatory responses in hiPSC-ECs and the analysis of leukocyte adhesion under physiological flow.

Tags

Keywords: MicrofluidicLeukocyte AdhesionHuman Induced Pluripotent Stem Cell-derived Endothelial CellsBiochip CoatingCell SeedingLive ImagingMicrofluidic Pump

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