Surface Modified 3d Scaffolds
Surface Modified 3d Scaffolds - Related Videos
08:06
Interview: Bioreactors and Surfaced-Modified 3D-Scaffolds for Stem Cell Research
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2008
In the past many in vitro culture systems -- mainly monolayer cultures -- often suffered from the disadvantage that differentiated primary cells had a relatively short life-span and de-differentiated during culture. As a consequence, most of their organ-specific functions were lost rapidly. Thus, in order to reproduce better conditions for these cells in vitro, modifications and adaptations have been made to conventional monolayer cultures.
09:34
Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
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Cited by 12 •
2017
Here, we present a protocol to visualize blood vessel formation in vivo and in real-time in 3D scaffolds by multiphoton microscopy. Angiogenesis in genetically modified scaffolds was studied in a murine calvarial critical bone defect model. More new blood vessels were detected in the treatment group than in controls.
07:04
Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
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Cited by 52 •
2012
This work details the preparation of 3D fibrin scaffolds for culturing and differentiating plutipotent stem cells. Such scaffolds can be used to screen the effects of various biological compounds on stem cell behavior as well as modified to contain drug delivery systems.
12:37
3D Hydrogel Scaffolds for Articular Chondrocyte Culture and Cartilage Generation
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Cited by 38 •
2015
Cartilage repair represents an unmet medical challenge and cell-based approaches to engineer human articular cartilage are a promising solution. Here, we describe three-dimensional (3D) biomimetic hydrogels as an ideal tool for the expansion and maturation of human articular chondrocytes.
14:49
Self-reporting Scaffolds for 3-Dimensional Cell Culture
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Cited by 7 •
2013
Biocompatible pH responsive sol-gel nanosensors can be incorporated into poly(lactic-co-glycolic acid) (PLGA) electrospun scaffolds. The produced self-reporting scaffolds can be used for in situ monitoring of microenvironmental conditions whilst culturing cells upon the scaffold. This is beneficial as the 3D cellular construct can be monitored in real-time without disturbing the experiment.
FAQs
Surface modifications influence cell behavior by changing how the scaffold interface interacts with proteins, cells, and biological fluids. Altered wettability, charge, roughness, ligand presentation, or coating composition can affect protein adsorption and, in turn, cell adhesion, proliferation, migration, and differentiation. These changes help researchers tune cellular responses without redesigning the entire scaffold.
Protein adsorption provides an important link between an engineered surface and cellular behavior. Changes in wettability, charge, roughness, or coating composition can alter which biological interactions occur at the interface. Because cells respond to this interface, surface engineering can influence adhesion and later outcomes such as proliferation, migration, and differentiation.
Tissue-specific responses can be encouraged by tuning the scaffold interface rather than changing its complete bulk structure. Presenting suitable ligands or adjusting surface properties may create interactions that support particular patterns of cell adhesion, proliferation, migration, or differentiation. This tunability is relevant when engineered tissues must provide more biologically appropriate cellular environments.
Researchers can adjust several interface characteristics, including wettability, surface charge, roughness, ligand presentation, and coating composition. Each property provides a different way to control interactions with cells and biological fluids. Selecting among these variables allows scaffold surfaces to be tailored toward improved biocompatibility or responses relevant to tissue formation.
In tissue engineering, these scaffolds provide organized three-dimensional environments in which researchers can study cell behavior and support tissue formation. Surface engineering adds control over the biological interface, which may improve biocompatibility and encourage tissue-specific responses. This combination makes the scaffolds useful for developing engineered tissues and investigating regenerative processes.
The approach is useful when researchers need a three-dimensional biomaterial environment with a tunable biological interface. In regenerative medicine, surface changes can support biocompatibility and tissue-specific responses. In drug testing, the organized scaffold environment can provide a setting for studying cell behavior. It is also relevant to developing engineered tissues.