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Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Preparation of 3D Fibrin Scaffolds for Stem Cell Culture Applications
DOI:

07:04 min

March 02, 2012

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Chapters

  • 00:05Title
  • 01:31Day One: Making Fibrinogen Solutions and Overnight Dialysis
  • 02:57Day 2: Polymerization of Fibrin Scaffolds and Seeding of Stem Cells into Scaffolds
  • 06:01Representative Fibrin Scaffold Results
  • 06:38Conclusion

Summary

Automatic Translation

This work details the preparation of 3D fibrin scaffolds for culturing and differentiating plutipotent stem cells. Such scaffolds can be used to screen the effects of various biological compounds on stem cell behavior as well as modified to contain drug delivery systems.

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