Murine Colon Cells
Murine Colon Cells - Related Videos
10:19
A Murine Model of Group B Streptococcus Vaginal Colonization
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Cited by 64 •
2016
The purpose of this protocol is to imitate human group B Streptococcus (GBS) vaginal colonization in a murine model. This method may be used to investigate host immune responses and bacterial factors contributing to GBS vaginal persistence, as well as to test therapeutic strategies.
06:16
Cryosectioning Method for Microdissection of Murine Colonic Mucosa
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Cited by 5 •
2015
Here we describe a simple method for the isolation of spatially distinct murine colonic epithelial surface cells from the underlying crypt-base cells.
08:52
RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
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Cited by 8 •
2011
A reliable method for the RNA isolation of Pseudomonas aeruginosa recovered from murine cecums is described. The RNA recovered is of sufficient quantity and quality for subsequent qPCR, transcription profiling, and RNA Seq experiments. This technique can be adapted for RNA isolation of other intestinal microbes.
03:18
Modeling Ovarian Cancer Cell Colonization of Omentum: An Ex Vivo Method to Establish Milky Spot Colonization of Fluorescently Labeled Ovarian Cancer Cells in Murine Omentum Explants
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2023
In this video, we establish an ex vivo omental organ culture overlaid with GFP-expressing ovarian cancer cells to study ovarian cancer colonization within the omental explant. This protocol analyzes immune cell structures within the omentum known as milky spots that promote metastases.
05:01
Lamina Propria Mononuclear Cell Isolation: A Silica-based Density Gradient Centrifugation Technique to Obtain Mononuclear Cells from Murine Colon
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2023
In this video, we demonstrate the isolation of lamina propria mononuclear cells from murine colonic tissue by enzymatic digestion using collagenase E and silica-based density separation media gradient separation. The isolated mononuclear cells can be used for subsequent downstream analysis.
FAQs
Stem-cell compartments support continual epithelial renewal, replacing cells as the lining changes. Tight junctions help regulate movement between neighboring epithelial cells, while mucus-producing goblet cells limit direct contact between luminal contents and underlying tissue. Together, these features support barrier function and help preserve intestinal homeostasis in the mouse colon.
Epithelial, immune, stromal, and neural populations provide complementary functions within the colon. Epithelial cells support the barrier, immune cells contribute to host defense, and stromal and neural populations add tissue-support and regulatory components. Studying these populations together is important because colon behavior reflects interactions among multiple cell types rather than epithelial activity alone.
The colon lining separates luminal contents and microbes from underlying tissue while participating in host defense. Examining epithelial barriers, mucus production, and associated immune responses can therefore connect cellular behavior with host-microbe interactions. Murine colon systems are useful for investigating how these relationships relate to intestinal homeostasis, inflammation, and epithelial repair.
Mouse studies connect cellular mechanisms with organism-level physiology, but their findings require careful comparison with human colon biology. A response observed in murine tissue, cultured cells, organoids, or a genetically modified model should therefore be interpreted as evidence from a mouse system, not automatically as a direct representation of human disease or treatment response.
Researchers examine these cells in tissue sections, primary cultures, organoids, and genetically modified mouse models. Tissue sections preserve anatomical context, whereas cultures and organoids support cellular investigation outside the intact organism. Genetically modified models add an organism-level approach for connecting altered cellular mechanisms with intestinal physiology and disease-related responses.
Tissue sections are appropriate when the investigation requires examination of murine colon cells within their native tissue context. Primary cultures and organoids provide complementary systems for studying cellular mechanisms outside the intact colon, while genetically modified mice support organism-level analysis. Comparing these formats can help relate localized cellular observations to broader biological responses.
Genetically modified mouse models allow researchers to examine how altered biology affects colon cells and intestinal physiology in vivo. They can be used alongside tissue sections, primary cultures, or organoids to investigate epithelial repair, inflammation, host-microbe interactions, colorectal disease, and responses to candidate therapies across different experimental levels.
These models support studies of epithelial repair, inflammation, host-microbe interactions, colorectal disease, and candidate-therapy responses. Their value comes from linking cell-level mechanisms, such as barrier maintenance or mucus-associated protection, with tissue and organism-level outcomes. Interpreting results across experimental formats also helps identify which findings depend on cellular context or intact physiology.