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A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast <em>Saccharomyces cerevisiae</em>
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae

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10:27 min

July 18, 2011

DOI:

10:27 min
July 18, 2011

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Summary

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A robust approach to monitor the delivery of organelles to the acidic lumen of the yeast vacuole for degradation and recycling is described. The method relies on the specific labeling of target organelles with a genetically encoded dual-emission fluorescence pH-biosensor, and visualization of individual cells using fluorescence microscopy.

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