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Chapters
- 00:05Title
- 01:33Preparation of Yeast Strains
- 03:00Cell Growth and Induction of Mitophagy by Nitrogen Starvation
- 04:48Labeling of the Vacuole with CMAC-Arg
- 06:26Mounting Live Yeast Cells for Fluorescence Microscopy
- 07:52Visualizing Mitophagy by Fluorescence Microscopy
- 10:02Conclusion
A robust approach to monitor the delivery of organelles to the acidic lumen of the yeast vacuole for degradation and recycling is described. The method relies on the specific labeling of target organelles with a genetically encoded dual-emission fluorescence pH-biosensor, and visualization of individual cells using fluorescence microscopy.
