Cancer Metabolism Studies
Cancer Metabolism Studies - Related Videos
07:29
Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
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Cited by 27 •
2015
Pancreatic cancer stem cells (CSCs) can be expanded in vitro using the anchorage-independent sphere culture technique, which represents a powerful tool to study CSC biology and can serve as the first step to develop novel CSC-targeting therapies. Here the methodology for expanding, analyzing and targeting of pancreatic CSCs is provided.
13:18
How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
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Cited by 4 •
2016
Here we explain a protocol for modelling the biophysical microenvironment where crosslinking and increased stiffness of the basement membrane (BM) induced by advanced glycation endproducts (AGEs) has pathological relevance.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
06:04
LC-MS Analysis of Human Platelets as a Platform for Studying Mitochondrial Metabolism
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Cited by 7 •
2016
Here we show isolated human platelets can be used as an accessible ex vivo model to study metabolic adaptations in response to the complex I inhibitor rotenone. This approach employs isotopic tracing and relative quantification by liquid chromatography-mass spectrometry and can be applied to a variety of study designs.
10:36
Systems Biology of Metabolic Regulation by Estrogen Receptor Signaling in Breast Cancer
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Cited by 5 •
2016
Integration of data from genome-wide sequencing experiments and metabolomics experiments is a challenge. In this paper we report, for the first time, generation, analysis and integration of transcriptome, cistrome and metabolome data from breast cancer cells treated with estradiol.
FAQs
Aerobic glycolysis can support malignant growth by reshaping how cells process nutrients and energy, even when mitochondrial respiration remains relevant. In cancer metabolism studies, researchers examine this pathway alongside glutamine utilization, lipid synthesis, and respiratory activity rather than treating it as an isolated feature. This broader comparison helps reveal which metabolic routes support tumor growth under particular conditions.
Oncogenic signaling and local nutrient availability can redirect metabolic networks, changing which substrates cancer cells use and which biosynthetic or energy-producing pathways they prioritize. Consequently, the same malignant cell population may display different metabolic dependencies in different environments. Studying these influences helps researchers distinguish stable vulnerabilities from adaptations driven by changing conditions.
Mitochondrial respiration and redox balance provide complementary perspectives on how tumor cells manage energy and cellular chemical conditions. Examining them with glycolysis, glutamine use, and lipid synthesis can show whether a metabolic state reflects energy production, biosynthetic demands, or adaptation. These relationships are important when evaluating vulnerabilities that might be disrupted therapeutically.
Metabolic adaptation may help malignant cells survive when treatment changes their demands for energy, biosynthetic materials, or redox control. Cancer metabolism studies investigate these responses by combining metabolic measurements with genetic perturbation and drug response assays. The resulting evidence can connect a resistance phenotype with a reshaped metabolic network and suggest which dependencies merit further evaluation.
Researchers commonly combine metabolic profiling, isotope tracing, genetic perturbation, and drug response assays. Metabolic profiling characterizes pathway-related changes, while isotope tracing follows how nutrients are used through cellular networks. Genetic perturbation tests the contribution of selected factors, and drug response assays examine how metabolic disruption relates to treatment effects. Together, these approaches provide complementary evidence rather than relying on one measurement.
Isotope tracing helps researchers follow the use and redirection of nutrients through cancer-associated metabolic networks. When interpreted alongside metabolic profiling, it can provide evidence about how cells allocate substrates toward energy production, biosynthesis, or other metabolic demands. This information helps clarify pathway activity and supports the search for dependencies that may not be apparent from abundance measurements alone.
Cancer metabolism findings can guide evaluation of strategies that disrupt tumor bioenergetics or biosynthesis. Researchers pair these strategies with drug response assays and other metabolic analyses to identify vulnerabilities and investigate treatment resistance. An important goal is to affect malignant-cell requirements while limiting effects on normal cells, making selectivity a central consideration in therapeutic development.