Cell Signal Transduction
Cell Signal Transduction - Related Videos
10:13
A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
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Cited by 6 •
2013
Achieving a systems level understanding of cellular processes is a goal of modern-day cell biology. We describe here strategies for multiplexing luciferase reporters of various cellular function end-points to interrogate gene function using genome-scale RNAi libraries.
12:24
Mimicking the Function of Signaling Proteins: Toward Artificial Signal Transduction Therapy
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2016
We present guidelines for developing synthetic 'chemical transducers' that can induce communication between naturally unrelated proteins. In addition, detailed protocols are presented for synthesizing and testing a specific 'transducer' that enables a growth factor to activate a detoxifying enzyme and consequently, to regulate the cleavage of an anticancer prodrug.
01:26
Signal Transduction: Overview
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2025
Cells respond to many types of information, often through receptor proteins positioned on the membrane. They respond to chemical signals, such as hormones, neurotransmitters, and other signaling molecules, initiating a series of molecular reactions to produce an appropriate response. This is called signal transduction. Cells also coordinate different responses elicited by the same signaling molecule via mediators, allowing molecular cross-talk. Typically, signal transduction involves three...
10:14
Retroviral Transduction of T-cell Receptors in Mouse T-cells
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Cited by 21 •
2010
We present a protocol to produce antigen-specific mouse T-cells using retroviral...
14:03
Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
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Cited by 8 •
2011
Lentiviruses are a valuable research tool for exploring gene function; however, researchers may wish to avoid production of pantropic lentivirus encoding known or suspected oncogenes. As an alternative, we present a safer protocol for use of ecotropic lentivirus on human cells modified to express the ecotropic receptor mSlc7a1.
FAQs
Receptors initiate signaling by recognizing specific external or internal cues, then pass information through linked intracellular components. These relay networks can activate second messengers, alter protein activity through phosphorylation, and ultimately influence gene expression. The sequence allows a signal detected at one location to produce coordinated changes in cell behavior, rather than an isolated molecular response.
Second messengers help distribute and amplify information within the cell after receptor activation. Protein phosphorylation changes the activity of signaling proteins, allowing successive steps in a relay network to be turned on or off. Together, these mechanisms regulate how strongly and how long a cell responds, which is important when researchers assess abnormal signaling in tumors.
Cancer-associated signaling abnormalities can make proliferative pathways remain active when they should be restrained. They may also support continued survival by reducing responses that normally promote cell death, while altering communication with surrounding tissue. Examining these changes helps distinguish signaling behavior that drives disease from regulated activity associated with normal growth, division, or differentiation.
Investigations commonly follow the pathway from receptor activity through second messengers and protein phosphorylation to changes in gene expression. Researchers can then relate these molecular changes to cellular outcomes such as proliferation, survival, division, or differentiation. Comparing signaling patterns with surrounding-tissue interactions may also reveal how abnormal communication contributes to disease behavior.
Abnormal pathway activity can provide molecular indicators of disease-driving processes. By identifying altered receptors, relay components, phosphorylation patterns, second-messenger activity, or signaling-related gene-expression changes, researchers can evaluate which features distinguish cancerous behavior. Such findings may support biomarker development by connecting measurable molecular changes with proliferation, survival, or other relevant cellular outcomes.
Mapping the steps that sustain oncogenic signaling can reveal points where a therapy might interrupt a disease-driving pathway. Researchers focus on abnormal receptor activity, intracellular relays, phosphorylation events, or downstream gene-expression effects rather than treating signaling as a single event. The resulting pathway knowledge supports strategies designed to reduce persistent proliferation or resistance to cell death.