Chronic Fibrosis
Chronic Fibrosis - Related Videos
15:43
Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice
0 Views •
Cited by 78 •
2014
We describe the agar-beads method to establish persistent long-term chronic Pseudomonas aeruginosa airway infection in the mouse model.
08:40
Chronic Salmonella Infection Induced Intestinal Fibrosis
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Cited by 4 •
2019
This protocol describes a mouse model of Salmonella driven intestinal fibrosis that resembles key pathological hallmarks of Crohn’s disease including transmural inflammation and fibrosis. This method can be used to evaluate host factors that alter fibrotic outcomes using mutant mice maintained on a C57Bl/6 genetic background.
09:12
A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia
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Cited by 3 •
2023
We established a mouse model of chronic liver fibrosis, which provides a suitable animal model for the virus-induced liver fibrosis mechanistic study of biliary atresia (BA) and a platform for future BA treatments.
08:56
Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis
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Cited by 300 •
2015
Disruption of bile flow results in severe inflammatory cholestatic liver injury with a characteristic time-dependent sequence of morphological alterations. Here we present a protocol for the surgical ligation of the common bile duct in mice that allows to induce a strong fibrotic response after 21 to 28 days.
06:28
An Obstructive Chronic Pancreatitis Model Established Through Electrocoagulation
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2025
The present protocol describes a novel approach to modeling chronic obstructive pancreatitis, which involves visualization of the pancreatic duct and electrocoagulation of the pancreatic duct to cause pancreatic duct obstruction.
FAQs
Persistent activation of fibroblasts and other stromal cells maintains excessive extracellular matrix deposition and remodeling. Collagen accumulation is therefore not an isolated structural change; it reflects continued activity within the tumor stroma. In cancer research, examining these cellular and matrix processes helps clarify how fibrotic tissue develops and remains established around tumors.
Dense fibrotic stroma can alter tissue mechanics and create a physical environment that restricts drug penetration. These changes are important because the amount of therapy reaching tumor-associated tissue may depend partly on matrix organization and stromal density. Studying this relationship connects fibrosis-associated structure with the practical challenges of evaluating cancer treatments.
The organization of extracellular matrix proteins, including collagen, helps determine how fibrotic stroma is arranged rather than merely how much matrix is present. Researchers examine this organization because it can influence tissue mechanics, drug penetration, and immune-cell infiltration. Matrix structure therefore provides a useful link between stromal biology and tumor microenvironment behavior.
Characterization can address several connected features: activation of fibroblasts and other stromal cells, accumulation and remodeling of extracellular matrix proteins, tissue mechanics, drug penetration, and immune-cell infiltration. Researchers also examine fibrosis-associated signaling pathways. Considering these features together provides a broader picture of how the tumor microenvironment changes during fibrotic development.
It is especially relevant when researchers are examining the tumor microenvironment, tumor progression, or the performance of therapies affected by stromal conditions. Fibrotic changes can influence tissue structure, drug access, and immune-cell entry. Consequently, fibrosis characterization supports studies that connect the physical and cellular properties of tumor-associated stroma with treatment evaluation.
Cancer studies can evaluate strategies directed at stromal cells, extracellular matrix organization, or fibrosis-associated signaling pathways. These approaches are examined because modifying fibrotic components may affect the tumor microenvironment, including its mechanics, drug penetration, or immune-cell infiltration. Such evaluations help determine how stromal targets relate to tumor progression and therapeutic response.