Cees Permeation
Cees Permeation - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
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2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
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Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
05:54
Controlled Odor Mimic Permeation Systems for Olfactory Training and Field Testing
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Cited by 1 •
2021
The Controlled Odor Mimic Permeation System is a simple, field-portable, low-cost method of odor delivery for olfactory testing and training. It is constructed of an odorant retained on an adsorbent material and contained inside of a permeable polymer bag allowing controlled release of the odorant vapor over time.
08:21
Permeabilization of Adhered Cells Using an Inert Gas Jet
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Cited by 3 •
2013
This protocol describes a method for the temporary permeabilization of adherent cells using an inert gas jet. This technique facilitates the transfer of genetic material and biomolecules into adherent mammalian cells by the utilization of mechanical forces to disrupt the plasma membrane.
08:35
Examining BCL-2 Family Function with Large Unilamellar Vesicles
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Cited by 24 •
2012
Biochemically-defined large unilamellar vesicles (LUVs) are a convenient model system to analyze BCL-2 family interactions with immediate implications in better understanding the mitochondrial pathway of apoptosis. A method to produce LUVs, along with standard BCL-2 family protein combinations and controls to examine LUV permeabilization, are presented.
FAQs
The process proceeds through three linked stages: CEES dissolves into the material, diffuses through its molecular structure down a concentration gradient, and desorbs at the opposite surface. Each stage contributes to whether the chemical crosses the barrier and becomes detectable on the far side. This sequence connects molecular transport with measurable barrier performance.
Temperature and exposure time can change how much CEES enters and moves through a material during testing. A longer exposure provides more opportunity for transport, while temperature alters the conditions governing movement within the material. Controlling and recording both variables is therefore important when comparing permeation behavior or evaluating chemical resistance.
Material chemistry determines how CEES interacts with the barrier and how readily it moves through the material's molecular structure. Thickness changes the distance that CEES must cross before reaching the opposite surface. Considering both properties helps explain differences in permeation rates among polymer films, coatings, and protective gloves.
A basic evaluation places a material barrier between CEES and the receiving side, allows exposure for a defined period, and examines whether CEES passes through to the opposite surface. The test should account for the material type, thickness, temperature, and exposure time. Comparing the resulting transport behavior supports assessment of barrier performance.
Measurements indicate how effectively a material resists CEES transport under specified exposure conditions. They can reveal differences in chemical resistance and barrier performance, especially when materials vary in chemistry or thickness. The results help characterize whether a polymer film, coating, or glove material is suitable for further chemical safety or hazard-mitigation evaluation.
CEES serves as a sulfur mustard simulant for studying chemical transport without focusing only on the finished protective product. In chemistry research, its movement through films, coatings, membranes, and gloves provides a way to examine interactions between a chemical and a material. Those findings support development and evaluation of protective clothing and related barriers.