Slc Superfamily
Slc Superfamily - Related Videos
07:49
Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
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2017
The goal of this protocol is to develop a reference for divergent proteins in a group that lacks coherent criteria for nomenclature and classification. This reference will facilitate analyses and discussion of the group as a whole and can be used in addition to established names.
06:36
Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization
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Cited by 50 •
2014
The zebrafish embryo is an excellent model for developmental biology research. During embryogenesis, zebrafish develop with a yolk mass, which presents three-dimensional challenges for sample observation and analysis. This protocol describes how to create two-dimensional flat mount preparations of whole mount in situ (WISH) stained zebrafish embryo specimens.
02:47
Protein Families
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2020
Protein families are groups of homologous proteins; that is, they have similarities in amino acid sequences and three-dimensional structures. Protein families usually occur because of gene duplication, where an additional copy of a gene is inserted into the genome of an organism. Mutations that change the amino acids but still allow the protein to be properly synthesized, will lead to new protein family members. If these new proteins contain similar amino acids in key locations, protein...
14:03
Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
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Cited by 8 •
2011
Lentiviruses are a valuable research tool for exploring gene function; however, researchers may wish to avoid production of pantropic lentivirus encoding known or suspected oncogenes. As an alternative, we present a safer protocol for use of ecotropic lentivirus on human cells modified to express the ecotropic receptor mSlc7a1.
14:06
Isolation of Precursor B-cell Subsets from Umbilical Cord Blood
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Cited by 2 •
2013
Here we describe a protocol for isolating subsets of precursor B-cells from umbilical cord blood. A sufficient quantity and quality of nucleic acids may be extracted from the cells and used in subsequent assays utilizing DNA or RNA.
FAQs
The relevant gradient determines whether a substrate moves with or against the combined chemical and electrical forces across a membrane. Facilitated diffusion follows an existing gradient, whereas secondary active transport uses an ion gradient to support movement that may not follow a substrate’s concentration gradient. This distinction helps explain how individual SLC proteins contribute to cellular homeostasis.
Exchange allows movement of one substrate to be linked to movement of another across the membrane. Rather than describing transport only as inward or outward flow of a single substance, this mechanism emphasizes coordinated substrate movement and helps maintain cellular balances. In clinical research, recognizing exchange behavior can clarify how altered transporter activity affects physiology or drug handling.
Changes in the amount or functional activity of an SLC protein can modify the movement of ions, metabolites, nutrients, or drugs across cell membranes. Such changes may disturb cellular homeostasis or alter exposure to therapeutic compounds. The overview connects these effects with inherited disorders, cancer, neurological disease, and drug resistance, making transporter regulation relevant to disease-mechanism studies.
Characterization can link a transporter’s expression or activity with the movement of clinically relevant substrates, including metabolites, nutrients, and drugs. This information helps researchers investigate how transport contributes to normal physiology or disease-associated changes. It also supports identification of potential biomarkers and improves interpretation of why treatment responses may differ between biological contexts.
Transporter studies help predict how drugs enter cells, move through biological systems, interact with other compounds, and influence treatment response. These predictions are clinically important because altered transporter activity can change drug handling and may contribute to resistance. Evaluating SLC-related transport therefore connects membrane biology with pharmacological decisions and the interpretation of therapeutic outcomes.
SLC-focused investigations can examine whether transporter changes are associated with inherited disease, cancer, neurological disease, or resistance to treatment. They can also evaluate whether transporter expression or activity serves as a biomarker or helps explain variable drug responses. This broad scope makes SLC research useful for connecting molecular transport processes with patient-relevant disease and therapy questions.