Endoparasitoid Wasps

Endoparasitoid wasps are insects whose larvae develop inside a living host, using the host’s tissues and resources until the host eventually dies; they provide a powerful system for studying animal development. A female wasp deposits an egg within or on a host, and the developing larva coordinates growth, tissue consumption, and protection from host immune defenses through interactions established during parasitism. In developmental biology, these wasps help researchers investigate embryogenesis, metamorphosis, host–parasite signaling, immune regulation, and resource allocation. Their specialized life cycle also informs biological control, because many species naturally suppress insect pests and may support sustainable pest-management strategies.

Endoparasitoid Wasps - Related Videos

Research

JoVE Journal - Developmental Biology

Protocols for Efficient Rearing and Functional Analysis of the Endoparasitoid Wasp Asobara japonica and its Host Drosophila melanogaster

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2026

The goal of this protocol is to facilitate functional analysis of parasitoid-host interactions in Asobara japonica and Drosophila melanogaster through optimized rearing methods, a single-oviposition infection assay, and RNAi-mediated gene knockdown.

An Introduction to Parasitic Wasps of Drosophila and the Antiparasite Immune Response

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Cited by 41 •

2012

Parasitoid (parasitic) wasps constitute a major class of natural enemies of many insects including Drosophila melanogaster. We will introduce the techniques to propagate these parasites in Drosophila spp. and demonstrate how to analyze their effects on immune tissues of Drosophila larvae.

Optimizing the Rearing Procedure of Germ-Free Wasps

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2023

Nasonia wasp embryos were dissected from Lucillia sericata pupae after parasitization for 12-24 h and washed with alcohol and 10% sodium hypochlorite solution to obtain germ-free embryos. After rearing the germ-free embryos and supplying them with Nasonia rearing medium to grow and develop in vitro, germ-free Nasonia adults were obtained.

Evaluation of the Productivity of Social Wasp Colonies (Vespinae) and an Introduction to the Traditional Japanese Vespula Wasp Hunting Technique

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Cited by 5 •

2019

This methodological paper evaluates the productivity of a social wasp colony by examining the number of meconia per 100 cells of comb, to estimate the total number of adults the wasps produced. The associated video describes how to search for Vespula wasp nests, a method developed by amateur wasp chasers.

Extracting Venom from the Parasitoid Wasp Trichogramma dendrolimi Using an Artificial Host

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Cited by 1 •

2023

Here, we present a protocol for extracting venom from Trichogramma dendrolimi using an artificial host created with polyethylene film and amino acid solution.

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