Extracting Venom from the Parasitoid Wasp <em>Trichogramma dendrolimi</em> Using an Artificial Host

Hai-yan Wang; Ze-qi Yu; Xin-yue Ren; Yuan-xi Li; Zhi-chao Yan

doi:10.3791/66032

Journal

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Biochemistry

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Extracting Venom from the Parasitoid Wasp Trichogramma dendrolimi Using an Artificial Host

Extracting Venom from the Parasitoid Wasp <em>Trichogramma dendrolimi</em> Using an Artificial Host

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Extracting Venom from the Parasitoid Wasp Trichogramma dendrolimi Using an Artificial Host

Extracting Venom from the Parasitoid Wasp Trichogramma dendrolimi Using an Artificial Host

1,410 Views

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03:53 min

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October 06, 2023

DOI:

10.3791/66032-v

DOI:

10.3791/66032-v

•

03:53 min

October 06, 2023

•

31 Views

Hai-yan Wang*^1,2, Ze-qi Yu*^1,2, Xin-yue Ren^1,2, Yuan-xi Li^1,2, Zhi-chao Yan^1,2,3

¹Department of Entomology,Nanjing Agricultural University, ²Key Laboratory of Integrated Pest Management of Crop of East China,Ministry of Agriculture, ³Sanya Institute of Nanjing Agricultural University

Transcript

The lack of efficient venom extraction methods often limits the research on the venom of parasitoid wasps, especially in tiny parasitoid wasps. This study provides an efficient method to extract the venom of Trichogramma with follow up post study of Trichogramma venom, such as the protein compensation and the venom function analysis. Previously, no methods of exchanging of parasitoid wasp venom requires the distinguishing of venom reserve.

However, parasitoid wasps are tiny. Not only are the technical requirements of distinguishing venom was high, but continuation of other tissues during dissection is also common. Our normal method uses artificial host, thus avoiding such problems.

In the future, we will focus on the agriculture and the medical applications of Trichogramma venom. We aim to utilize specific insecticidal venom genes for pest control. Meanwhile, we plan to investigate the untapped potential of Trichogramma venom in methodology, particularly its prospective utility in enhancing immunotherapy techniques, and in the discovery of novel antibiotic molecules.

Summary

Here, we present a protocol for extracting venom from Trichogramma dendrolimi using an artificial host created with polyethylene film and amino acid solution.