Swine Ischemia Model
Swine Ischemia Model - Related Videos
08:22
A Chronic Cardiac Ischemia Model in Swine Using an Ameroid Constrictor
0 Views •
Cited by 14 •
2017
The purpose of this protocol is to demonstrate the placement of a delayed constricting device (an ameroid constrictor) around a coronary artery in a swine model. This device creates an ischemic area of the heart that is useful for studying new diagnostic imaging techniques and new methods of treatment.
12:51
Murine Model of Hindlimb Ischemia
0 Views •
Cited by 201 •
2009
The surgical procedure for induction of unilateral hindlimb ischemia is demonstrated, with confirmation of ischemia by laser Doppler perfusion imaging.
09:24
An Anoxia-starvation Model for Ischemia/Reperfusion in C. elegans
0 Views •
Cited by 4 •
2014
A protocol is described that uses anoxia/starvation in C. elegans to model ischemia/reperfusion. Functional outcomes include increased mortality, visible abnormalities in GFP-labeled neuronal processes, and impaired behavioral responses that require neuronal function.
10:05
Testing the Efficacy of Pharmacological Agents in a Pericardial Target Delivery Model in the Swine
0 Views •
Cited by 4 •
2016
We have developed a swine model for the target delivery of pharmacological agents within the pericardial space/fluid. Using this approach, the relative benefits of administered agents on induced atrial fibrillation, relative refractory periods and/or ischemic protection can be investigated.
07:07
Murine Model of Intestinal Ischemia-reperfusion Injury
0 Views •
Cited by 52 •
2016
Here we describe the detailed procedure of intestinal ischemia-reperfusion in mice which results in reproducible injury without mortality to encourage the standardization of this technique across the field. This model of intestinal ischemia-reperfusion injury can be utilized to study the cellular and molecular mechanisms of injury and regeneration.
FAQs
Limiting blood flow restricts delivery of oxygen and glucose, forcing cells to alter their metabolism. During ischemia, this metabolic disturbance can progress to tissue damage; when circulation returns, the reperfusion phase provides a separate context for examining how injured tissue responds and recovers. This distinction helps researchers connect perfusion failure with cellular survival and organ injury.
Restoring circulation after an ischemic period can produce biological effects distinct from those caused by reduced blood flow alone. Studying the two phases allows researchers to examine both the initial consequences of limited oxygen and glucose delivery and the tissue response during recovery. This separation is important when evaluating injury progression, cellular survival, and protective interventions.
In developmental biology, the model can be used to examine how impaired circulation affects tissue growth, vascular development, cellular survival, and recovery. These outcomes connect blood-flow disturbances with the processes that build and maintain developing tissues. The system therefore supports investigation of how vascular insufficiency may alter developmental trajectories as well as later tissue repair.
The experimental design centers on inducing or monitoring a defined interruption of perfusion in a pig. Researchers can then examine tissue responses during the reduced-flow period and after circulation resumes. Keeping the perfusion change defined helps relate altered oxygen and glucose delivery to subsequent metabolic changes, tissue damage, cellular survival, and recovery.
Researchers may use this model to study disease mechanisms and to evaluate protective interventions or regenerative strategies under ischemic and reperfusion conditions. Its value lies in connecting a controlled perfusion disturbance with measurable biological consequences in tissues and organs. This makes it relevant for asking whether an approach supports survival, limits injury, or promotes recovery.
Swine provide anatomical and physiological similarities to humans, giving ischemia studies a physiologically relevant experimental context. Those similarities support investigation of how reduced perfusion affects organs and tissues while allowing researchers to examine injury, protection, and regeneration in an intact organism. In developmental biology, the same context strengthens studies of circulation-dependent growth and vascular development.