Fetal Brain Collection
Fetal Brain Collection - Related Videos
14:26
Progenitor-derived Oligodendrocyte Culture System from Human Fetal Brain
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Cited by 38 •
2012
Primary, human fetal brain-derived, multipotential progenitor cells proliferate in vitro while maintaining the capacity to differentiate into neurons and astrocytes. This work shows that neural progenitors can be induced to differentiate through stages of the oligodendrocytic lineage by conditioning with select growth factors.
05:44
Concurrent Collection of Fetal Murine Brain and Serum to Assess Effects of Maternal Diet on Nutrition and Neurodevelopment in Neurofibromatosis Type 1
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Cited by 1 •
2024
In this protocol, we describe a method for simultaneous collection of fetal brain tissue as well as high-quality, non-hemolyzed serum from the same mouse embryo. We have utilized this technique to interrogate how maternal dietary exposure affects macronutrient profiles and fetal neurodevelopment in mice heterozygous for Nf1 (Neurofibromatosis Type 1).
12:28
Abbiategrasso Brain Bank Protocol for Collecting, Processing and Characterizing Aging Brains
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Cited by 25 •
2020
This protocol describes a method to track individual brain-aging trajectories through a brain donation program and proper characterization of brains. Brain donors are involved in a long-term longitudinal study including serial multi-dimensional assessments. The protocol contains a detailed description of brain processing and an accurate diagnostic methodology.
07:06
Collection of Frozen Rodent Brain Regions for Downstream Analyses
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Cited by 20 •
2020
This procedure describes the collection of discrete frozen brain regions to obtain high-quality protein and RNA using inexpensive and commonly available tools.
08:04
Murine Fetal Echocardiography
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Cited by 7 •
2013
Fetal and perinatal death is a common feature when studying genetic alterations affecting cardiac development. High-frequency ultrasound imaging has improved 2-D resolution and can provide excellent information on early cardiac development and is an ideal method to detect the impact on cardiac structure and function prior to death.
FAQs
The preservation condition should match the intended analysis. Fixation or freezing can be selected to maintain the cellular or nucleic-acid features needed by a study, rather than treating every specimen identically. This decision matters because later work may focus on tissue characteristics, DNA-based genotyping, or RNA-based gene-expression patterns, each requiring material preserved for its specific purpose.
DNA and RNA answer different genetic questions. DNA supports genotyping, allowing investigators to examine inherited genetic information, whereas RNA supports gene-expression analysis and investigation of regulatory changes active during neural development. Selecting the appropriate nucleic acid therefore links collection and preservation choices to the study's central objective, whether it concerns inherited disorders or changing developmental programs.
Anatomical dissection provides the step that isolates the brain from the developing fetus before preservation and molecular work. Keeping this anatomical target explicit helps align the sample with questions about neural development, brain formation, and brain function. In genetics research, that alignment is important because the resulting DNA or RNA measurements are interpreted in a brain-specific developmental context.
Under an approved research protocol, the workflow proceeds from retrieval and anatomical isolation to preservation in a condition suited to the planned study. The preserved tissue can then undergo DNA or RNA extraction for downstream analysis. This sequence connects the physical sample to genotyping, gene-expression measurement, or investigation of regulatory changes while emphasizing protection of cellular and nucleic-acid integrity.
Researchers may use it to investigate inherited disorders, developmental biology, and the genetic pathways that shape brain formation and function. Because the sample comes from a developing brain, analyses can examine genetic information alongside expression or regulatory changes associated with neural development. The approach therefore connects tissue-level sampling with questions about how genetic pathways influence the developing brain.
It can provide DNA-based genotyping results, RNA-based gene-expression measurements, and evidence relevant to regulatory changes during neural development. Together, these outputs help researchers examine inherited disorders and the pathways involved in brain formation and function. Interpretation remains tied to the preservation and analysis conditions used, since those conditions are selected to maintain the material required for each measurement.