Recombinant Parasites
Recombinant Parasites - Related Videos
13:39
Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites
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Cited by 9 •
2011
Genetic crosses of rodent malaria parasites are performed by feeding two genetically distinct parasites to mosquitoes. Recombinant progeny are cloned from mouse blood after allowing mosquitoes to bite infected mice. This video shows how to produce genetic crosses of Plasmodium yoelii and is applicable to other rodent malaria parasites.
08:56
Recombineering and Gene Targeting
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2023
One of the most widely used tools in modern biology is molecular cloning with restriction enzymes, which create compatible ends between DNA fragments that allow them to be joined together. However, this technique has certain restrictions that limit its applicability for large or complex DNA construct generation. A newer technique that addresses some of these shortcomings is recombineering, which modifies DNA using homologous recombination (HR), the exchange between different DNA molecules based...
14:23
Recombineering Homologous Recombination Constructs in Drosophila
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Cited by 9 •
2013
Homologous recombination techniques greatly advance Drosophila genetics by enabling the creation of molecularly precise mutations. The recent adoption of recombineering allows one to manipulate large pieces of DNA and transform them into Drosophila6. The methods presented here combine these techniques to rapidly generate large homologous recombination vectors.
03:29
An In Vitro Assay to Quantify the Intracellular Growth of Parasite in Macrophages
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2025
This video demonstrates an assay to quantify the intracellular growth of parasites in bone marrow-derived macrophages (BMMs). The macrophage cells are infected with the motile and non-motile invasive stages of Leishmania, metacyclic promastigotes and amastigotes, respectively. The parasites invade the macrophages, getting internalized into a parasitophorous vacuole and multiplying inside. The intracellular parasite growth is quantified using a microscope upon fluorescently staining the...
02:33
A Technique to Develop a Parasite Infection in Intestinal Organoids via Microinjection
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2025
This video demonstrates a microinjection technique to develop a human intestinal organoid infection model. The infectious sporozoite stage of the parasite Cryptosporidium parvum is microinjected into the organoid lumen. Upon invading the epithelial cells of the organoid, it undergoes asexual and sexual reproduction to complete its life cycle and spreads the infection.
FAQs
Targeted modification depends on delivering engineered DNA or gene-editing components into parasite cells and directing repair at a defined genomic site. Homologous recombination can integrate a desired sequence through the corresponding repair process, while CRISPR-based repair provides another route for introducing the planned change. This precision helps researchers relate a specific genotype to an observed phenotype.
They make modified cells easier to identify after genetic material or editing components have been delivered. A selectable marker or reporter gene acts as an indicator that helps researchers distinguish candidate modified lines from cells that lack the intended change. This step supports establishment of lines suitable for downstream genetic and host–parasite studies.
Changing one defined sequence creates a way to test its functional contribution rather than merely observing that the sequence is present. Researchers can then examine how the alteration affects parasite biology or host–parasite interactions. In genetics, this design links genotype with phenotype and supports more direct interpretation of a gene or sequence’s role.
An experimental workflow begins by delivering engineered DNA or gene-editing components into parasite cells. Researchers use homologous recombination or CRISPR-based repair to integrate the planned change at a defined genomic site. Selectable markers and reporter genes can then help identify modified lines, creating experimental systems for functional gene analysis and host–parasite interaction studies.
Researchers can modify parasite sequences and examine the biological characteristics associated with the resulting genetic change. This makes the system useful for testing whether particular alterations are linked to drug resistance or virulence. The approach supports functional gene analysis within a parasite-based context, helping connect molecular changes to traits relevant to infection and treatment.
By enabling functional analysis of parasite genes, recombinant parasite models can help evaluate candidate vaccine or therapeutic targets. They also provide genetic systems for examining host–parasite interactions and may contribute to disease surveillance and antiparasitic treatment development. Their value lies in testing how defined genetic changes relate to biologically relevant parasite traits.