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High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
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Neuroscience
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JoVE Journal Neuroscience
High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity

High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity

DOI:
10.3791/51640-v

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12:02 min

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June 02, 2014

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, ,
1Monell Chemical Senses Center

Chapters

  • 00:05Title
  • 01:28Plating Cells for Transfection
  • 02:57Transfection of Olfactory Receptors
  • 04:52Odor Stimulation
  • 05:52Measuring Olfactory Receptor Activity via Luciferase Assay
  • 09:50Results: Characterization of Odorant/Olfactory Receptor Interactions
  • 11:27Conclusion

Summary

Automatic Translation

Automatic Translation

Olfactory receptor activation patterns encode odor identity, but the lack of published data identifying odorant ligands for mammalian olfactory receptors hinders the development of a comprehensive model of odor coding. This protocol describes a method to facilitate high-throughput identification of olfactory receptor ligands and quantification of receptor activation.

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Olfactory ReceptorsHigh-throughput ScreeningLuciferase Reporter AssayOdorant-receptor InteractionsOdor CodingMammalian Olfactory System

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