Gut Analysis
Gut Analysis - Related Videos
15:27
Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
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Cited by 17 •
2007
This video illustrates the technique for extracting DNA from the species of microbes resident in the termite hindgut. The preparation of a wet mount slide, which is useful for visualizing the gut microbial community is also illustrated, and a tour through the species-rich gut environment is given.
02:46
Investigating the Human Gut Microbial Activity on Exopolysaccharides
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2026
Source: Hu, Y. et.al., Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems. J. Vis. Exp. (2019)This video demonstrates the analysis of short-chain fatty acid production by human gut microbiota in response to exopolysaccharides. This highlights microbial fermentation products separation and quantification using gas chromatography. The results reveal that exopolysaccharides modulate microbial metabolites that influence host-pathogen...
10:17
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
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Cited by 6 •
2017
Most common gut content analyses of macroinvertebrates are visual. Requiring intense knowledge about morphological diversity of prey organisms, they miss soft bodied prey and, due to strong comminution of prey, are nearly impossible for some organisms, including amphipods. We provide detailed, novel genetic approaches for macroinvertebrate prey identification in the diet of amphipods.
07:54
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
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Cited by 9 •
2017
Bacteriocins are believed to play a key role in defining microbial diversity in different ecological niches. Here, we describe an efficient procedure to assess how bacteriocins affect gut microbiota composition in an animal model.
10:25
Competitive Homing Assays to Study Gut-tropic T Cell Migration
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Cited by 11 •
2011
Competitive homing experiments allow to directly assessing the migratory properties of two different cell populations in a single mouse. Here we illustrate this procedure by comparing the migration of ex vivo-generated gut-tropic versus non-gut tropic T cells.
FAQs
Its central value is connecting three observations: the condition of the gut barrier, the organisms present, and the host response to them. A barrier-related change can alter pathogen colonization, while microbial communities may influence immune defense and disease progression. Examining these features together therefore provides more context than studying infection or inflammation as an isolated finding.
These methods answer complementary questions. Microbial DNA sequencing profiles the organisms or microbial communities present, microscopy provides visual examination of stool or tissue, and immunoassays measure host-response signals such as cytokine or antibody activity. Combining their outputs links microbial composition with physical findings and immune activity, producing a broader picture of intestinal health or disease.
Organism detection alone does not show how the host is responding. Cytokine and antibody measurements add evidence of immune activity, helping researchers assess whether intestinal changes are associated with a detectable host response. When interpreted with microbial and tissue findings, these signals can clarify relationships among infection, inflammation, immune defense, and disease progression.
Stool and tissue provide complementary sources of information. Stool can be examined for microbial communities and their DNA, while tissue permits direct microscopic assessment of intestinal material and related changes. Immunoassays can then measure host-response markers in the investigation. Selecting and combining these sample types allows microbial, structural, and immune findings to be considered together.
The resulting profiles can describe differences in microbial communities, intestinal findings, and immune activity associated with disease. Repeating or comparing these profiles during treatment evaluation may show whether those features change alongside the intervention. This approach gives researchers several types of evidence for characterizing disease and assessing whether treatment is associated with altered microbial or immune patterns.
In this field, the approach helps investigate how intestinal microbes and the gut barrier influence pathogen colonization, immune defense, and disease progression. It can also support research aimed at restoring microbial and immune balance. By relating organism profiles to cytokine or antibody activity, investigators can study infection within the broader context of host-microbe interactions.