Immune Cell Engraftment
Immune Cell Engraftment - Related Videos
04:36
Adenocarcinoma Engraftment: A Method to Implant Cancer Cells into the Murine Lungs
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2023
This video describes a method to engraft lung adenocarcinoma cells in murine lungs by preconditioning the airways with fibrosis-inducing agent–bleomycin. This method significantly enhances orthotopic engraftment of lung cancer cells into murine...
09:39
Normal and Malignant Muscle Cell Transplantation into Immune Compromised Adult Zebrafish
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Cited by 16 •
2014
Here, we present a protocol for cell transplantation of zebrafish skeletal muscle and embryonal rhabdomyosarcoma (ERMS) into adult immune compromised rag2E450fs homozygous mutant zebrafish. This protocol allows for the efficient analysis of regeneration and malignant transformation of muscle cells.
10:52
Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
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Cited by 1 •
2018
Mantle cell lymphoma (MCL) is a difficult to treat B cell disorder and it is equally difficult to establish a xenograft mouse model of primary MCL to study and develop therapeutics. Here, we describe the successful establishment of MCL xenografts in mice to help understand its underlying biology.
07:26
Isolation of Functional Cardiac Immune Cells
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Cited by 9 •
2011
This method for isolating functional immune cells from the heart provides an alternative to the conventional methods of collagenase digestion, which causes unwanted immune cell activation, resulting in a decreased responsiveness of these cells. Our method of isolation yields functional cardiac immune cells by avoiding problems associated with enzymatic digestion.
15:39
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
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Cited by 8 •
2019
Herein we describe two approaches to characterize cell polarization events in B lymphocytes during the formation of an IS. The first, involves quantification of organelle recruitment and cytoskeleton rearrangements at the synaptic membrane. The second is a biochemical approach, to characterize changes in composition of the centrosome, which undergoes polarization to the immune synapse.
FAQs
Successful immune cell engraftment depends on more than cell presence alone. Transferred or newly generated cells must migrate to appropriate tissue niches, survive through interactions with local signals, and remain capable of responding to antigenic stimulation and cytokines. These linked requirements help explain why tissue location, persistence, and responsiveness are evaluated together rather than as isolated features.
Antigenic stimulation and cytokines influence what happens after cells reach a suitable niche. They can support expansion, function, or both, making engraftment more than a measurement of persistence. By examining these responses under defined experimental conditions, investigators can distinguish a population that remains present from one that actively contributes to immune defense or develops a harmful immune response.
Measuring survival, tissue distribution, lineage, and function provides complementary evidence about an engrafted population. Survival indicates whether cells persist, distribution shows whether they occupy appropriate tissues, lineage identifies the type of immune cells present, and function reveals their activity. Together, these measurements connect cellular persistence with immune reconstitution and defense-related outcomes.
An evaluation can follow a straightforward sequence: identify the transferred or newly generated cell population, examine its survival and tissue distribution, determine its lineage, and assess its function. The final interpretation should consider antigenic stimulation and cytokine responses alongside persistence. This workflow links where cells are found with what they do under defined experimental conditions.
Immune Cell Engraftment models provide a way to examine host-pathogen interactions in a system with defined immune-cell contributions. Investigators can ask whether established cells persist in appropriate tissues, respond to antigenic stimulation, and contribute to protective or harmful immune responses. These measurements help connect cellular behavior with infection-related immune outcomes.
These models support studies of immune reconstitution and cellular therapies by tracking whether transferred or newly generated cells survive, where they distribute, which lineage they represent, and whether they function after establishment. Such information can clarify how immune systems recover under defined conditions and whether the resulting response contributes to protection or becomes potentially harmful.