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A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)

A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)

DOI:
10.3791/60460-v

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10:41 min

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April 30, 2020

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, , , , , , , ,
1Division of Infection and Global Health, School of Medicine,University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences,University of Glasgow

Chapters

  • 00:05Introduction
  • 01:33Sample Preparation and Heat Inactivation
  • 02:30RNA Extraction
  • 06:28Reverse Transcriptase qPCR
  • 07:57Results: Heat Inactivation of M. tuberculosis Bacilli
  • 09:43Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a tuberculosis molecular bacterial load assay test performed after heat inactivation of sputum. Heat inactivation renders sputum samples noninfectious and obviates the need for containment level 3 laboratories for tuberculosis molecular tests.

Tags

Keywords: Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)TuberculosisDisease BurdenAnti-tuberculosis MedicationTreatment ResponseQuantitative ResultNontuberculous MycobacteriaChronic Obstructive Pulmonary DiseaseBacille Calmette-Guerin (BCG)Sputum SamplesRNA ExtractionPhenol ChloroformExtraction Control

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