Real-time Electrophysiology
Real-time Electrophysiology - Related Videos
09:13
Remote Magnetic Navigation for Accurate, Real-time Catheter Positioning and Ablation in Cardiac Electrophysiology Procedures
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Cited by 51 •
2013
This report provides a detailed description of a new remote navigation system based on magnetic driven forces, which has been recently introduced as a new robotic tool for human cardiac electrophysiology procedures.
08:08
Real-time Electrophysiology: Using Closed-loop Protocols to Probe Neuronal Dynamics and Beyond
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Cited by 10 •
2015
Closed-loop protocols are becoming increasingly widespread in modern day electrophysiology. We present a simple, versatile and inexpensive way to perform complex electrophysiological protocols in cortical pyramidal neurons in vitro, using a desktop computer and a digital acquisition board.
08:16
Movement Retraining using Real-time Feedback of Performance
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Cited by 4 •
2013
Retraining abnormal movement patterns following injury or disease is a key component of physical rehabilitation. Recent advances in technology have permitted accurate assessment of movement during a variety of tasks, with near instantaneous quantification of results. This provides new opportunities for modification of faulty movement patterns in real time.
05:41
Applying Microfluidics to Electrophysiology
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Cited by 1 •
2007
Microfluidics can be integrated with standard electrophysiology techniques to allow new experimental modalities. Specifically, the motivation for the ...
02:57
Real Time RT-PCR
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2021
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction. The real-time quantification of the number of amplified products is...
FAQs
It can track membrane potential, ion currents, and changes in conductance as cells respond over time. These measurements distinguish shifts in the electrical state of a cell from broader observations of activation, helping researchers connect immune stimulation or inflammatory signaling with changes in ion-channel activity and cellular function.
Millisecond-scale measurements capture rapid electrical changes that may be missed by slower methods. Following membrane dynamics during stimulation allows researchers to relate the timing of ion currents or conductance changes to an immune-cell response, pathogen exposure, or inflammatory signal, strengthening links between an external event and its immediate cellular effects.
Patch-clamp and extracellular recording provide complementary ways to monitor electrical behavior in living cells or tissues. Using these approaches, investigators can examine changes in membrane potential, ion currents, or conductance while a biological stimulus is present. The selected recording strategy therefore helps match the measurement to the electrical feature being followed.
A basic workflow is to establish an electrical recording from living cells or tissue, observe the changing signal, introduce or monitor a relevant stimulus, and compare the electrical response over time. In immunology and infection studies, the stimulus may involve immune-cell activation, pathogen exposure, or inflammatory signaling, with the resulting traces interpreted alongside cellular function.
Measurements can show whether immune-cell activation is accompanied by altered membrane dynamics, ion currents, or conductance. This electrical information adds a functional layer to studies of signaling and host defense, helping researchers determine how activation-associated signals affect cellular behavior rather than treating activation as an isolated molecular event.
When cells encounter pathogens or inflammatory signals, real-time electrophysiology can identify changes in electrical activity and ion-channel behavior that accompany dysfunction. Such findings help connect pathogen-driven effects with altered cellular signaling and host defense, while also highlighting electrical processes that may represent potential therapeutic targets.