Viral Rna Transfection
Viral Rna Transfection - Related Videos
10:16
Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Cited by 35 •
2012
A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.
05:38
RNA Extraction Assay: A Method to Extract RNA from miRNA Transfected Lung Cancer Cells
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2023
This video describes the RNA extraction protocol from miRNA transfected lung cancer cells. This approach can be used to identify a combinatorial miRNA treatment activity in halting the cell cycle and angiogenesis.
09:35
Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
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Cited by 15 •
2013
This method describes the use of click chemistry to measure changes in host cell transcription after infection with the Rift Valley fever virus (RVFV) strain MP-12. Results can be visualized qualitatively via fluorescence microscopy or obtained quantitatively through flow cytometry. This method is adaptable for use with other viruses.
10:15
Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
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Cited by 5 •
2017
Next generation electroporation is an efficient method for transfecting human Th17 cells with small RNAs to alter gene expression and cell behavior.
08:04
Neuronal Transfection Methods
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2023
Transfection - the process of transferring genetic material into cells - is a powerful tool for the rapid and efficient manipulation of gene expression in cells. Because this method can be used to silence the expression of specific proteins or to drive the expression of foreign or modified proteins, transfection is an extremely useful tool in the study of the cellular and molecular processes that govern neuron function. However, mature neurons have a number of properties that make them...
FAQs
Positive-sense viral genomes can serve directly as templates for translation once they reach the cytoplasm, allowing viral proteins to be produced without an additional copying step. Other viral RNA types cannot necessarily function this way and may depend on complementary polymerases or helper components for replication and gene expression. This distinction determines which experimental components are needed to initiate infection-related processes.
Lipid-based reagents and electroporation provide different ways to place purified viral RNA inside host cells, with the cytoplasm serving as the relevant destination for subsequent viral activity. The delivery approach therefore affects whether the RNA reaches the cellular compartment where translation, replication, or gene expression can occur. Comparing these approaches can help researchers establish an effective experimental introduction method.
Once viral RNA enters host cells, the resulting cellular responses can be examined alongside viral replication and gene expression. In immunology and infection research, this makes the approach useful for studying innate immune sensing, the early host response that detects infection-related molecular signals. These experiments help connect viral RNA activity with host defense mechanisms and broader host-pathogen interactions.
Reverse-genetics systems use introduced viral RNA to analyze how specific viral genes contribute to replication or infection-related behavior. By working with viral genomes or engineered genome versions, researchers can connect genetic features with observed viral functions. This provides a framework for investigating viral gene activity and for examining how changes in the genome influence interactions between the virus and host cell.
A general workflow begins with purified viral RNA and a method such as a lipid-based reagent or electroporation to introduce it into host cells. The RNA then reaches the cytoplasm, where its behavior depends on viral RNA type and available polymerase or helper components. Researchers can subsequently examine translation, replication, gene expression, or associated cellular responses.
The method is useful when researchers need to examine viral replication, innate immune sensing, or host-pathogen interactions in host cells. It also supports reverse-genetics experiments that investigate viral genes and their functions. Because the approach introduces viral genetic material directly, it provides a way to focus experiments on genome activity and the cellular consequences associated with infection-related processes.
Results from these experiments can clarify how viral genomes initiate replication-related activity, express genes, and interact with host defenses. That information can guide antiviral development by identifying infection-associated processes for investigation. The same research context can inform RNA-based vaccine platform design, particularly by improving understanding of how introduced viral or viral-related RNA behaves in host cells.