Autologous Graft
Autologous Graft - Related Videos
09:30
Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation
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Cited by 44 •
2016
The protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.
10:44
Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
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Cited by 60 •
2012
Clinically relevant animal models of intracerebral hemorrhage (ICH) are needed to extend our knowledge of hemorrhagic stroke and to examine novel therapeutic strategies. In this study, we describe and evaluate two ICH models that implement unilateral injections of either autologous whole blood or bacterial collagenase into the basal ganglia (corpus striatum) of mice.
07:37
Mouse Models for Graft Arteriosclerosis
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Cited by 17 •
2013
We describe protocols for our mouse graft arteriosclerois (GA) models which involve interposition of a mouse vessel segment into a recipient of the same inbred strain. By backcrossing additional genetic changes into the vessel donor, the model can assess the effect of specific genes on GA.
10:30
Mesenchymal Stromal Cell Culture and Delivery in Autologous Conditions: A Smart Approach for Orthopedic Applications
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Cited by 28 •
2016
Culturing human Mesenchymal Stromal Cells (hMSCs) with autologous serum, reduces the risks of rejection by xenogeneic material and other negative effects. It also allows for the recovery of a subset of mesodermal progenitors, which can deliver fresh hMSCs. Embedding hMSCs in an autologous fibrin clot enables easy handling and effective surgical implantation.
08:58
Matrix-assisted Autologous Chondrocyte Transplantation for Remodeling and Repair of Chondral Defects in a Rabbit Model
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Cited by 5 •
2013
An experimental technique for the treatment of chondral defects in the rabbit's knee joint is described. The implantation of autologous chondrocytes seeded on a matrix is a well-accepted method for the remodeling and repair of articular cartilage lesions providing satisfying long-term results. Matrix-assisted autologous chondrocyte transplantation (MACT) offers a standardized and clinically established implantation method.
FAQs
Genetic identity between graft and recipient reduces immune incompatibility, which lowers the risk that the transferred tissue will be rejected. This advantage does not eliminate the need for careful surgical planning: harvested tissue must remain viable, and the recipient site must support blood supply and cellular integration. Thus, compatibility improves the biological starting conditions for healing.
Blood supply helps sustain transferred tissue; cellular integration helps it become established at the recipient site; remodeling supports longer-term healing and structural adaptation. If any of these processes is inadequate, an otherwise compatible graft may fail. Their progression therefore links early viability with eventual repair, making the recipient environment as important as the graft’s genetic compatibility.
Despite its compatibility advantage, this approach can injure the donor site, and the amount of suitable tissue may be limited. Those constraints can affect whether enough material can be harvested and add another area requiring healing. Consequently, selecting an autologous graft requires balancing the expected benefit at the recipient site against tissue availability and donor-site consequences.
Preparation begins with harvesting suitable tissue from the patient, followed by preserving its viability. Surgeons then place it into a prepared recipient site, where blood supply, cellular integration, and tissue remodeling support healing. This sequence emphasizes that transfer alone is not the endpoint: both tissue handling and recipient-site preparation contribute to whether the graft becomes established and supports repair.
Reported examples include skin, bone, blood vessels, tendons, and fat. Their use reflects the broad range of tissues that can be transferred for reconstruction, wound coverage, or repair after injury or disease. The relevant choice depends on having suitable tissue available for harvest and matching the graft’s role to the needs of the recipient site.
Clinical applications described for this approach include reconstruction, coverage of wounds, and repair after injury or disease. A graft may also contribute to restoring or supporting a damaged tissue site through integration and remodeling. Its medical relevance comes from combining these repair goals with reduced immune incompatibility, while clinicians must still account for donor-site injury, limited availability, and possible graft failure.