Chimeric Mouse Model
Chimeric Mouse Model - Related Videos
10:39
Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
0 Views •
Cited by 1 •
2015
There is an overall lack of knowledge about how vaccines work. Here we propose the combined use of reverse genetics and bone marrow chimeric mice to gain insight into the early host immune responses to vaccines with a special focus on dendritic cells and T cell immunity.
21:01
Generating Chimeric Zebrafish Embryos by Transplantation
0 Views •
Cited by 61 •
2009
A step-by-step guide to generating targeted chimeric zebrafish embryos by transplantation at the blastula or gastrula stage.
09:11
A Mouse Model of in Utero Transplantation
0 Views •
Cited by 26 •
2011
The mouse model of in utero transplantation is a versatile tool that can be used to study the potential clinical applications of stem cell transplantation and gene therapy in the fetus. In this protocol, we present a general approach to performing this...
10:56
A Familial Hypercholesterolemia Human Liver Chimeric Mouse Model Using Induced Pluripotent Stem Cell-derived Hepatocytes
0 Views •
Cited by 5 •
2018
Here, we present a protocol to generate a human liver chimeric mouse model of familial hypercholesterolemia using human induced pluripotent stem cell-derived hepatocytes. This is a valuable model for testing new therapies for hypercholesterolemia.
05:53
Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates
0 Views •
Cited by 1 •
2018
Here we present a protocol to increase chimera production without the use of new equipment. A simple orientation change of the embryo for injection can increase the number of embryos produced, and potentially reduce the timeline to germline transmission.
FAQs
Impaired immune function reduces rejection of engrafted human or other donor-derived material, giving the graft an opportunity to develop or respond in the mouse. This condition is therefore central to studying human cells, tumors, or tissues in vivo. Without limiting rejection, the donor-derived component may not remain available for meaningful biological or therapeutic analysis.
The engrafted material sets the biological question the model can address. Human hematopoietic stem cells can support investigation of blood-related and immune processes, whereas tumors are suited to cancer research, and tissues or organs can provide other human biological contexts. Selecting the donor component accordingly helps align the model with disease mechanisms, biomarkers, or treatment-response questions.
These systems place selected human biology within a living mouse, but mouse physiology does not fully reproduce human biology. A disease response, biomarker pattern, or therapeutic effect observed after engraftment may therefore reflect both the donor-derived component and the host environment. The models are valuable for preclinical reasoning, yet they do not eliminate the need for careful interpretation.
At a high level, researchers select a donor-derived component that matches the scientific question, use mice with impaired immune function to reduce rejection, and engraft human hematopoietic stem cells, tumors, or tissues. They then examine how the graft develops or responds in the living animal, including disease features, biomarkers, or therapeutic effects relevant to the study.
They are used across cancer, infectious disease, immune-mediated disease, and transplantation research. The same general platform can also support therapeutic response studies and biomarker research, because donor-derived material can be observed in a living organism. Its value depends on matching the engrafted component to the medical problem under investigation.
By placing human-derived cells, tumors, or tissues in a living host, the model allows investigators to examine selected therapeutic responses in an organismal setting. Such observations can contribute to preclinical drug evaluation and help connect treatment effects with disease mechanisms or biomarkers. Results remain translational evidence rather than a complete prediction of human outcomes because host physiology differs.
Human-derived biological material can provide a context for examining how disease-related systems respond to treatment. In medicine, those response patterns may help researchers investigate biomarkers and inform individualized treatment strategies. The model does not guarantee that a response will match a patient’s outcome, since it reproduces selected features of human biology rather than the whole human condition.