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Intranasal Administration of Recombinant Influenza Vaccines in Chimeric Mouse Models to Study Mucosal Immunity
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Chapters
- 00:05Title
- 01:49Generation of Recombinant Live Attentuated Influenza Vaccines by Reverse Genetics
- 04:03Inoculating Eggs and Harvesting Allantoic Fluid
- 05:02Tracking Vaccine-Specific Immunity: Peptide-Bearing Dendritic Cells
- 06:06Tracking Vaccine-Specific Immunity: Vaccine-Specific T Cell Proliferation
- 07:05Mouse Irradiation, Transplantation, Vaccination and Targeted Depletion
- 08:24Results: Modulation of Mucosal Immunity by Live Attentuated Virus; Role of CD103+ Dendritic Cells in Vaccine Induced Immunity
- 10:01Conclusion
There is an overall lack of knowledge about how vaccines work. Here we propose the combined use of reverse genetics and bone marrow chimeric mice to gain insight into the early host immune responses to vaccines with a special focus on dendritic cells and T cell immunity.
