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May 29, 2018
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Here we present a protocol to increase chimera production without the use of new equipment. A simple orientation change of the embryo for injection can increase the number of embryos produced, and potentially reduce the timeline to germline transmission.
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Cite this Article
Scott, G. J., Gruzdev, A., Hagler, T. B., Ray, M. K. Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates. J. Vis. Exp. (135), e56955, doi:10.3791/56955 (2018).
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