Intestinal Injury Assessment
Intestinal Injury Assessment - Related Videos
07:07
Murine Model of Intestinal Ischemia-reperfusion Injury
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Cited by 52 •
2016
Here we describe the detailed procedure of intestinal ischemia-reperfusion in mice which results in reproducible injury without mortality to encourage the standardization of this technique across the field. This model of intestinal ischemia-reperfusion injury can be utilized to study the cellular and molecular mechanisms of injury and regeneration.
02:45
Assessment of Intestinal Permeability in C. elegans Following Bacterial Exposure
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2026
Source: Le, T. A. N., et al., Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans. J. Vis. Exp. (2019)This video demonstrates a fluorescence-based assay to quantify intestinal permeability in C. elegans exposed to Pseudomonas aeruginosa. Compared to controls fed Escherichia coli, pathogen-treated worms show increased fluorophore leakage into the body cavity, indicating epithelial barrier damage.
02:28
Assessing Transcytosis of E. coli Across Intestinal Epithelial Monolayers
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2025
Source: Islam, A., et al., Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates. J. Vis. Exp. (2023)This video demonstrates the procedure for assessing transcytosis of neonatal Escherichia coli across intestinal epithelial monolayers using transwell inserts, electrical resistance measurement, and bacterial quantification.
06:04
Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
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Cited by 41 •
2016
This protocol describes the use of excised intestinal tissue preparations or "intestinal sacs" as an ex vivo model of intestinal barrier function. This model may be used to assess integrity of both the epithelial barrier and the mucous gel layer at specific intestinal sites in animal models of digestive disease.
09:44
Functional Assessment of Intestinal Motility and Gut Wall Inflammation in Rodents: Analyses in a Standardized Model of Intestinal Manipulation
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Cited by 32 •
2012
Postoperative ileus (POI) is a complication of abdominal surgery leading to increased morbidity and a prolonged hospital stay. Because prophylactic or therapeutic strategies are lacking intensified research is necessary. Therefore we established a standardized and feasible mouse model to investigate the pathophysiology of POI and to study potential therapeutic options.
FAQs
Barrier integrity reflects how well the intestinal lining maintains separation between the intestinal contents and underlying tissue. Epithelial disruption can therefore indicate structural damage even when broader clinical findings are limited, while impaired integrity may accompany inflammation, ulceration, or bleeding. Assessing this feature helps connect microscopic or endoscopic changes with functional consequences and mucosal healing.
These tools provide complementary evidence rather than interchangeable results. Endoscopy can document visible mucosal changes, imaging can contribute information about intestinal injury, histology examines tissue alterations, and biomarkers provide measurable indicators associated with damage or inflammation. Combining their findings gives clinicians a broader basis for judging severity, tracking change, and evaluating treatment response.
The distinction depends on relating findings to time and recovery. A single evaluation can document the current state, but repeated clinical assessments, targeted tests, or biomarker measurements can show whether abnormalities resolve or remain. Persistent epithelial disruption, inflammation, ulceration, bleeding, or barrier impairment may indicate ongoing injury, whereas improving measurements support recovery.
Intestinal injury assessment can place observed tissue and functional changes in the context of infection, ischemia, inflammation, or therapy-related toxicity. No single finding necessarily establishes the cause; clinicians interpret clinical information alongside endoscopic, imaging, histological, and biomarker results. This integrated approach helps connect disease manifestations with the processes producing intestinal damage.
The process begins with clinical findings and the suspected nature of the intestinal problem. Clinicians then select targeted evaluations, which may include endoscopy, imaging, histological examination, or biomarkers, and interpret the results together. Reassessment can compare current abnormalities with earlier findings, helping determine severity, support treatment decisions, and monitor recovery or continuing injury.
Multiple methods are useful when clinicians need information about different aspects of intestinal damage. Endoscopy, imaging, tissue examination, and biomarkers can address visible injury, broader intestinal changes, microscopic alterations, or measurable biological signals. Using several approaches is particularly relevant when evaluating severity, clarifying whether injury is acute or persistent, or judging response to treatment.
In clinical medicine, standardized measurements can contribute to diagnosis, prognosis, treatment evaluation, and monitoring of mucosal healing. In research, they allow tissue changes and functional impairment to be related to specific causes and to tested interventions. This makes assessment useful for studying strategies intended to preserve intestinal function and promote recovery after injury.